中国药理学通报Issue(11)：1526-1529,4.DOI:10.3969/j.issn.1001-1978.2014.11.009
CD44单抗A3 D8对NB4细胞IL-3 Rα及其下游信号通路的调节
Regulatory effects of CD44 antibody-A3 D8 on IL-3 Rαand downstream PI3 K/Akt pathway in NB4 cells
摘要
Abstract
Aim To investigate the effect of CD44 anti-body-A3 D8 on the expression of IL-3 Rα and down-stream PI3K/Akt in NB4 cells. Methods The ex-pression of IL-3 Rα mRNA was detected by real-time quantitative RT-PCR, the IL-3Rα protein expression and changes of PI3 K/Akt signal pathway in NB4 cells treated with A3D8 were analyzed by Western blot. An-nexin-V-FITC/PI double staining flow cytometry was u-tilized to detect the apoptotic cells. The inhibitor of PI3 K/Akt signaling LY294002 combined with A3 D8 was used to inhibit the PI3K/Akt in NB4 cells. Re-sults After treated with A3 D8 , both the transcription-al level and translational level of IL-3 Rα were remark-ably reduced, and the PI3K/Akt pathway was inhibi-ted. LY294002 improved the inhibitory and apoptotic effects of A3D8 on NB4 cells. Conclusion CD44 antibody A3 D8 can downregulate the expression of IL-3Rα and inhibit the downstream PI3K/Akt pathway.关键词
CD44/A3D8/NB4/IL-3Rα/PI3K/Akt 通/路/LY294002Key words
CD44/A3 D8/NB4/IL-3 Rα/PI3 K/Akt pathway/LY294002分类
医药卫生引用本文复制引用
陈萍,原琴,姜熙,伍娟英,黄慧芳..CD44单抗A3 D8对NB4细胞IL-3 Rα及其下游信号通路的调节[J].中国药理学通报,2014,(11):1526-1529,4.基金项目
国家自然科学基金资助项目( No 81100382) （ No 81100382）
福建省教育厅科技项目( No JB11059) （ No JB11059）
国家和福建省临床重点专科建设资助项目 （）
