中国药理学通报Issue(11)：1574-1579,6.DOI:10.3969/j.issn.1001-1978.2014.11.019
慢性间歇性低压低氧增强丹皮酚舒张大鼠动脉的作用
Chronic intermittent hypobaric hypoxia enhances vasodilative effects of paeonol on isolated thoracic aorta rings of rats
摘要
Abstract
Aim To investigate the effect of chronic intermittent hypobaric hypoxia ( CIHH) on the paeonol induced vasomotion of isolated rat ’ s thoracic aorta rings and its underlying mechanisms. Methods Spra-gue-Dawlay ( SD ) rats were randomly divided into 2 groups: control group ( CON ) and CIHH treatment group ( CIHH) . CIHH rats were exposed to hypoxia in a hypobaric chamber simulating 5 000 m altitude, 6 hours daily for 28 days. CON rats lived in the same en-vironment as CIHH animals except hypoxia. Organ bath technique was used to observe the effect of pae-onol on isolated thoracic aorta rings of rats. Results There were no significant differences of noradrenaline ( NE )- and KCl-induced contraction in thoracic aorta rings among CIHH and CON rats;CIHH enhanced va-sodilative effects of paeonol on isolated thoracic aorta rings of rats; the vasodilative effects on CIHH rats could be partly decreased by β-receptor blocker prop-ranolol,ATP-sensitive potassium channel ( KATP ) bloc-ker glibenclamide and NO synthase inhibitor L-NAME. Paeonol significantly inhibited NE-induced intracellular and extracellular calcium-dependent contraction in CIHH rats. Paeonol didn ’ t inhibit NE-induced con-traction by intracellular calcium release and its inhibi-tory effect couldn ’ t be blocked by glibenclamide in CON. Vasodilative effects of paeonol couldn ’ t be re-versed by indomethacin, a cyclooxygenase inhibitor, in CIHH and CON rats. Conclusion CIHH significantly enhances vasodilative effects of paeonol on isolated tho-racic aorta rings of rats. Besides promoting the signa-ling pathway of paeonol in CON, CIHH significantly enhances vasodilative effects of paeonol via activating KATP and inhibiting Ca2+ release from sarcoplasmic re-ticulum.关键词
慢性间歇性低压低氧/丹皮酚/胸主动脉环/血管舒张/ATP敏感钾通道/大鼠Key words
chronic intermittent hypobaric hypoxia ( CIHH)/paeonol/thoracic aortic rings/vasodilation/ATP-sensitive potassium channel/rat分类
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郭赞,宋士军,宋爽,马坤,于雷,宋彦丽,马会杰,张翼..慢性间歇性低压低氧增强丹皮酚舒张大鼠动脉的作用[J].中国药理学通报,2014,(11):1574-1579,6.基金项目
国家重点基础研究发展计划(973计划)资助项目( No 2006CB5041006) （973计划）
河北省科技资助计划( No 11246125D) （ No 11246125D）
河北省卫生厅资助项目( No 20110042) （ No 20110042）
河北省教育厅项目(No 2011154) （No 2011154）
