中国药理学通报Issue(12)：1671-1676,6.DOI:10.3969/j.issn.1001-1978.2014.12.011
冰片对鼻咽癌细胞容积敏感性氯通道的激活作用
Borneol activated volume-sensitive chloride channels in poorly differentiated nasopharyngeal carcinoma cells
摘要
Abstract
Aim To clarify the effect of Borneol on the chloride channels and cell volume in poorly differentia-ted nasopharyngeal carcinoma CNE-2Z cells.Methods The technique of whole-cell patch clamp was used to detect the chloride currents and analyze the character-istics of the currents in CNE-2Z cells.The volume changes caused by Borneol were measured by the meth-od of time-lapse live cell imaging.Results The chlo-ride currents were induced by extracellular application of Borneol (20 μmol·L -1 )isotonic condition.The currents showed a characteristic of outward rectification and did not show voltage-dependent or time-dependent inactivation.The reversal potential of the currents was close to the CI-equilibrium potential. The currents were inhibited by the chloride channel blocker tamox-ifen.The currents were also inhibited by 47% hyper-tonic solution.Borneol decreased the cell volume by 9.4% in 30 min.Tamoxifen completely inhibited the Borneol-induced cell volume decrease.Conclusion Borneol can activate volume-sensitive chloride channels and induce volume decrease in CNE-2Z cells.Chloride channels play a pivotal role in the process of volume decrease caused by Borneol.关键词
冰片/氯通道/氯通道阻断剂/癌细胞/膜片钳技术/细胞体积Key words
Borneol/chloride channels/chloride channel blocker/tumor cells/patch clamp technique/cell volume分类
医药卫生引用本文复制引用
孟龙,陈丽新,王立伟,王海波,邓志钦,汪源,伍嘉宝,赖周毅,吕瑞玲,孙晓雪,朱林燕..冰片对鼻咽癌细胞容积敏感性氯通道的激活作用[J].中国药理学通报,2014,(12):1671-1676,6.基金项目
国家自然科学基金资助项目（No 81273539，81173064）；教育部基金项目（No 20124401110009）；广州市科技计划基金项目（No 2013J450015）；东莞科技计划基金项目 （）
