中国药理学通报Issue(12)：1677-1680,1681,5.DOI:10.3969/j.issn.1001-1978.2014.12.012
牡荆素减轻小鼠溃疡性结肠炎的药效作用及机制研究
Effects and mechanisms of vitexin against ulcerative colitis in mice
摘要
Abstract
Aim To evaluate the effect and mecha-nism of vitexin in a mouse model of DSS-induced ulcer-ative colitis (UC).Methods C57BL/6 mice were randomly placed into three groups: normal control group,DSS group and DSS +Vitexin group.Mice coli-tis was induced by adding 4% dextran sulphate sodium (DSS)into the drinking water for seven days.Vitexin was administered once a day along with DSS treatment. Mice were monitored daily with body weight change and diarrhea symptoms.After sacrifice,colon was re-moved and fixed in 1 0% (W/V)buffered formalin for hematoxylin-eosin (H&E)staining.Histological dam-age was assessed as a combined score of inflammatory cell infiltration and mucosal damage.The remaining colon pieces were collected to measure the activity of myeloperoxidase (MPO)by ELISA method and to de-termine the mRNA expression of TNF-α,IL-6 and COX-2.Results None of the mice receiving vehicle alone exhibited body weight loss and mucosal disrup-tion at any point during the study.Vitexin treatment significantly ameliorated DSS-induced body weight loss and histological score.The activity of MPO and the mRNA expression of TNF-α,IL-6 and COX-2 were markedly inhibited by vitexin treatment.Conclusion Vitexin ameliorates DSS-induced colitis through sup-pressing leukocyte infiltration and pro-inflammatory mediators production.关键词
牡荆素/溃疡性结肠炎/葡聚糖硫酸钠/MPO/TNF-α/IL-6/COX-2Key words
vitexin/ulcerative colitis/dextran sul-phate sodium/MPO/TNF-α/IL-6/COX-2分类
医药卫生引用本文复制引用
孙阿宁,任改艳,邓超,张晶晶,王峥涛,窦薇..牡荆素减轻小鼠溃疡性结肠炎的药效作用及机制研究[J].中国药理学通报,2014,(12):1677-1680,1681,5.基金项目
国家自然科学基金资助项目（No 81273572）；上海市教委科研创新项目（No 13YZ043）；上海市自然科学基金资助项目 （）
