中国药理学通报Issue(12)：1692-1697,6.DOI:10.3969/j.issn.1001-1978.2014.12.015
无创肢体缺血预适应对心肌缺血／再灌注损伤内皮功能的影响
Effects of non-invasive limb ischemic preconditioning on endothelial function after myocardial ischemia-reperfusion injury
摘要
Abstract
Aims To investigate the protective effects of noninvasive limb ischemic preconditioning (LIPC) on myocardial ischemia-reperfusion (I /R)injury,and to explore the mechanism.Methods Healthy male Wistar rats were divided randomly into I /R,I /R +LIPC,I /R +5-Hydroxydecanoate (5-HD)and I /R +LIPC +5-HD groups.The I /R +LIPC and I /R +LIPC-5-HD groups of rats were subjected to three cy-cles of LIPC induction per day with 5 min of reperfu-sion after occlusion for 5 min at the left hind limb for 3 days.All rats were subjected to myocardial I /R injury on the fourth day.The I /R +5-HD and I /R +LIPC-5-HD groups of rats were given the inhibitor of ATP-sen-sitive potassium channel 5-HD before and during myo-cardial I /R injury. Results Compared with I /R group,LIPC reduced myocardial infarct size (P <0.05),lowered cardiocyte apoptosis index and Fas, FasL positive cell number (P <0.01 ),increased the reduced nitric oxide (NO)/endothelin (ET)-1 ratio (P <0.05)in serum in I /R +LIPC group.5-HD a-bolished the protective effects induced by LIPC in I /R+LIPC-5-HD group.Compared with normal myocardi-al tissue,expression of mir-30a-3p was increased in I /R group (P <0.01 )and was decreased in LIPC group (P <0.01 ).Conclusion LIPC alleviates myocardial I /R injury and improves endothelial function. The mechanism may be related with the opening of ATP-sensitive potassium channel,regulating the balance be-tween NO and ET-1 and decreasing the expression of myocardial mir-30a-3p.关键词
肢体缺血预适应/心肌缺血/再灌注/ATP 敏感性钾通道/一氧化氮/内皮素-1/mir-30a-3pKey words
limb ischemic preconditioning/myocardi-al ischemia-reperfusion/ATP-sensitive potassium channel/nitric oxide/endothelin-1/mir-30a-3p分类
医药卫生引用本文复制引用
陈伟佳,刘东明,强兆艳,温克,袁恒杰,康毅,娄建石,吴艳娜..无创肢体缺血预适应对心肌缺血／再灌注损伤内皮功能的影响[J].中国药理学通报,2014,(12):1692-1697,6.基金项目
国家自然科学基金资助项目（No 81102446，81173058，81102447）；天津市自然科学基金资助项目（No 10JCZDJC20900，11 JCYBJC14900）；天津市高等学校国家级大学生创新创业训练计划 （）
