中国药理学通报Issue(12)：1709-1712,1713,5.DOI:10.3969/j.issn.1001-1978.2014.12.018
昆明山海棠内生真菌ThF11化学成分抗菌杀线虫作用的研究
Antibacterial and nematicidal activity of endophyte ThF-11 of Tripterygium hypoglaucum (Levl.)Hutch
摘要
Abstract
Aim To investigate the chemical compo-nents and antibacterial and nematicidal activity of en-doparasitic fungi ThF-1 1 of Tripterygium hypoglaucum (Levl.)Hutch.Methods The endophyte ThF-1 1 was isolated,purified and identified based on fungal ITS sequence.The chemical components were isolated and identified with column chromatography and NMR spec-trometer,respectively.The nematicidal and antibacte-rial activity was investigated by biological active meth-od and inhibition zone method.Results The endo-phyte ThF-1 1 was identified as Penicillium simplicissi-mum,the compounds were identified as Penicillic acid [1 ],2,4-dimethoxy-6-methylphenol [2]and 5 (6)-dihydropenicillic acid [3 ] based on spectral data. Compound [1 ]showed significant activity against Cae-norhabditis elegans in a dose-dependent manner,and had antibacterial activity to Escherichia coli,Bacillus cereus,Bacillus subtilis, Staphylococcus aureus and Pseudomonas aeruginosa.Conclusion These com-pounds are firstly isolated from endophyte ThF-1 1 of T. hypoglaucum (Levl.)Hutch.Compound [1 ]shows strong nematicidal activity and antibacterial activity, and is worth further studying and developing.关键词
昆明山海棠/内生真菌/杀线虫/抑菌/简青霉/化学成分/药用植物Key words
T.hypoglaucum (Levl.)Hutch/endo-phyte/nematicidal/antibacterial/Penicillium simpli-cissimum/chemical component/medicinal plant分类
医药卫生引用本文复制引用
郑喜,李国红,王芯,祁燕,万春平..昆明山海棠内生真菌ThF11化学成分抗菌杀线虫作用的研究[J].中国药理学通报,2014,(12):1709-1712,1713,5.基金项目
国家自然科学基金资助项目（No 81360543）；云南省教育厅科学研究项目重点项目 （）
