中国药理学通报Issue(12)：1721-1724,1725,5.DOI:10.3969/j.issn.1001-1978.2014.12.020
内质网应激介导白藜芦醇对心肌细胞肥大的保护作用
Protection action of resveratrol on cardiomyocyte hypertrophy mediated by endoplasmic reticulum stress
摘要
Abstract
Aim To explore the role of resveratrol (Res)on cardiomyocyte hypertrophy induced by iso-proterenol (ISO)and the relationship with endoplas-mic reticulum stress (ERS).Methods Hypertrophic model of cardiomyocytes was induced by ISO.Hyper-trophy status of cardiomyocytes was determined by measuring the cell surface area and the gene expression of ANP.The value of apoptosis was measured by flow cytometry,the content of LDH and MDA was measured in different groups,and the gene and protein expres-sions of GRP78 and CHOP were detected by real-time PCR and Western blot.Results Res could attentuate ISO-induced cardiomyocyte hypertrophy and apoptosis by reducing the cell surface area,the gene expression of ANP and the value of apoptosis.Res could inhibit ERS by downregulating the gene and protein expression of GRP78 and CHOP.Meanwhile,the content of LDH and MDA was decreased.Conclusions The results suggest that treatment of Res may protect cardiomyocyte hypertrophy,which is partially mediated by inhibiting the expression of ERS factors GRP78 and CHOP.关键词
心肌肥大/内质网应激/白藜芦醇/细胞凋亡/GRP78/CHOPKey words
cardiac hypertrophy/endoplasmic reticu-lum stress/resveratrol/apoptosis/GRP78/CHOP分类
医药卫生引用本文复制引用
林岩,肖薇,金莉,邓志会,李波,王国忠,刘吉成..内质网应激介导白藜芦醇对心肌细胞肥大的保护作用[J].中国药理学通报,2014,(12):1721-1724,1725,5.基金项目
国家自然科学基金资助项目（No 81100170）；黑龙江省教育厅面上项目（No 12521617）；齐齐哈尔医学院科研基金 （）
