中国药理学通报Issue(12)：1725-1729,5.DOI:10.3969/j.issn.1001-1978.2014.12.021
丙泊酚对癫痫大鼠学习记忆及海马 TLR4表达的影响
Effects of propofol on learning and memorizing ability and hippocampus TLR4 in epileptic rats
摘要
Abstract
Aim To explore the effects of propofol on learning and memorizing ability and the effects of com-bination of propofol and phenobarbital sodium on epi-leptic rats.Methods Thirty-six epileptic rats were di-vided into epileptic model group (EP),normal saline group (NS),lipid emulsion +epileptic group (LE), phenobarbital sodium +epileptic group (PB),propofol+epileptic pattern (Prof),and combination of propo-fol and phenobarbital sodium +epileptic group.Each group had 6 rats.Tests of Morris water maze were giv-en to the rats to evaluate their learning and memorizing ability.The protein expression of toll-like receptor 4 (TLR4 )was examined by ELISA.Results There were no effects of saline and lipid emulsion on learning and memorizing ability and the expression of TLR4 pro-tein in hippocampus in epileptic rats (P >0.05 ). Propofol could increase the incubation period in epilep-tic rats obviously,shorten the plateau period,and in-crease the expression of TLR4 protein in hippocampus (P <0.05 ).Phenobarbital sodium could shorten the plateau period in epileptic rats,and increase the ex-pression of TLR4 protein in hippocampus (P <0.05), but it had no effect on the incubation period (P >0.05).Compared with PB,combination of propofol and phenobarbital sodium +epileptic group had a lon-ger incubation period and a shorter plateau period with an increase of the expression of TLR4 protein in hippo-campus (P <0.05 ).Compared with propofol group, combination of propofol and phenobarbital sodium +ep-ileptic group had a shorter plateau period (P <0.05) with an obvious increase in the expression of TLR4 pro-tein in hippocampus (P <0.05),but it had no effect on incubation period (P >0.05 ).Conclusions Propofol damages the learning and memorizing ability of epileptic rats.Phenobarbital sodium had no obvious effect on the learning ability in epileptic rats,but harms the memorizing ability in epileptic rats.Combi-nation of propofol and phenobarbital sodium affects the learning and memorizing ability of epileptic rats.Hip-pocampus TLR4 may be involved in the effect of propo-fol and phenobarbital sodium on the learning and mem-orizing ability of epileptic rats.关键词
癫痫/丙泊酚/学习记忆/海马/苯巴比妥钠/水迷宫Key words
epilepsia/propofol/learning and memori-zing ability/hippocampus/phenobarbital sodium/wa-ter maze分类
医药卫生引用本文复制引用
宋炜,蔡鹏,杜雷,王志达,许志强,张咏梅..丙泊酚对癫痫大鼠学习记忆及海马 TLR4表达的影响[J].中国药理学通报,2014,(12):1725-1729,5.基金项目
国家自然科学基金资助项目（No 81171041）；江苏省自然科学基金资助项目（No BK2011197）；江苏省高校自然科学研究重大项目（No 13KJA320001）；江苏省高等学校大学生实践创新训练计划项目（No 201310313035Y）。 （）
