中国药理学通报Issue(12)：1769-1773,5.DOI:10.3969/j.issn.1001-1978.2014.12.030
淫羊藿、黄芪、葛根有效组分复方对阿尔采末病转基因动物模型的学习记忆能力及病理学影响
Effects of effective fraction of Epimedium,Astragalus,Radix Puerariae on behavioral and pathological changes in a transgenic mouse model of Alzheimer’s disease
摘要
Abstract
Aim To observe the effects of effective fraction of Epimedium,Astragalus,Radix Puerariae on behavioral and pathological changes in a transgenic mouse model of Alzheimer’s disease.Methods Six-month-old APPswe /PS1 ΔE9 transgenic mice were ran-domly divided into 2 groups:model group and effective fraction group,1 0 mice each group.The mice in the effective fraction group were treated with the effective fraction of Astragalus,Radix Puerariae,Epimedium compound for 8 weeks.The C57BL/6J mice were used as negative control group.After 8 weeks,the learning and memory function were measured by Morris water maze,the pathological changes in brain tissue were ob-served by Modified Bielschowsky staining and Nissl 's staining.Results During place navigation trial,the escape latency in the APPswe /PS1 ΔE9 double transgenic model mice was longer than those of the mice of C57 (P <0.05),the escape latency in the mice of effec-tive fraction group was significantly reduced than those of the mice in model group (P <0.05).During spatial probe trail,the platform-crossing times in the APPswe /PS1 ΔE9 double transgenic mice were different from the mice of C57 (P <0.05),the platform-crossing times in the mice of effective fraction group were significantly increased than those of the mice in model group (P <0.05).The average swimming velocity in the APPswe /PS1 ΔE9 double transgenic model mice was increased than that of mice of C57 (P <0.05 ),there was no significant difference between effective fraction group and model group (P > 0.05 ). The Modified Bielschowsky staining shows that the neuron fibers of the cerebral cortex of APPswe /PS1 ΔE9 double transgenic mice were enlarged,swelling,and dense.There were senile plaques and nerve fiber tangles in the cerebral cortex of APPswe /PS1 ΔE9 double transgenic mice.The neuron fibers of mice in the effective fraction group were relieved;there was a small amount of senile plaque.The Nissl’s staining shows that the neurons of the cerebral cortex of APPswe /PS1 ΔE9 mice were edema, the number of cells were decreased.The mice in the effective fraction group were free of the disease.Con-clusion The double transgenic APPswe /PS1 ΔE9 mice of AD can simulate the specific pathogenesis of AD, which may be the efficient experimental animal model. The effective fraction of epimedium,astragalus and ra-dix puerariae may have a neuroprotective effect against AD via improving the learning and memory ability,and reduce the cerebral cortex nerve fiber tangles,senile plaques and neurons edema changes.关键词
阿尔采末病/转基因动物模型/淫羊藿/黄芪/葛根/Bielschowsky 银染/尼氏染色Key words
Alzheimer’s disease/transgenic mouse model/Epimedium/Astragalus/Radix Puerariae/Bielschowsky/Nissl′s staining分类
医药卫生引用本文复制引用
董贤慧,高维娟,孔卫娜,张瑜,邵铁梅,于文国,柴锡庆..淫羊藿、黄芪、葛根有效组分复方对阿尔采末病转基因动物模型的学习记忆能力及病理学影响[J].中国药理学通报,2014,(12):1769-1773,5.基金项目
国家自然科学基金资助项目（No 81273983）；河北省自然科学基金资助项目（No C2010001471）；河北省高等学校科学技术研究青年基金资助项目（No Q2012036）；河北省食品药品监督管理局资助项目 （）
