中国药理学通报Issue(1)：45-49,5.DOI:10.3969/j.issn.1001-1978.2015.01.011
人参总皂苷增强成骨分化的间充质干细胞促造血作用研究
Total saponins of panax ginseng enhances the effect of osteoblast differentiation from mesenchymal stem cell on promoting hematopoiesis
摘要
Abstract
Aim To observe mesenchymal stem cell differentiation into osteoblast induced by TSPG, and explore how TSPG enhances the promotion of hemato-poiesis of osteoblast differentiation from mesenchymal stem cell. Methods MSCs were cultured by TSPG combined with osteoinductive medium. The cellular vi-ability of proliferation was detected with MTT assay. The content of alkaline phosphatase in the cultural su-pernatant was tested with pNPP assay. The ability of MSCs to form calcium nodes was also observed after a-lizarin red stain. The protein expression of RUNX2 was analyzed with Western blot. The content of cytokines associated with hematopoiesis was tested with Elisa as-say. The ability of promoting hematopoiesis was detec-ted with hematopoietic colony forming assay. Results Both MTT and pNPP assay showed that optical den-sity ( OD) values were increased in response to TSPG treatment in a dose-dependent manner. The mineraliza-tion formation ability was enhanced with TSPG-treat-ment. Meanwhile, the expression of RUNX2 protein was up-regulated in TSPG-treated cell. Moreover, the content of cytokines associated with hematopoiesis and the number of hematopoietic progenitor colony were in-creased by TSPG-treatment compared with the control group. Conclusion TSPG could induce MSCs differ-entiation in to osteoblast and enhance the effect of oste-oblast differentiation from MSCs on promoting hemato-poiesis.关键词
人参总皂苷/间充质干细胞/成骨细胞/造血微环境/分化/造血Key words
total saponins of panax ginseng/mesen-chymal stem cell/osteoblast/hematopoietic microenvi-ronment/differentiation/hematopoiesis分类
医药卫生引用本文复制引用
尹利明,赵燕娜,杜文喜,王潇,沃立科,高瑞兰..人参总皂苷增强成骨分化的间充质干细胞促造血作用研究[J].中国药理学通报,2015,(1):45-49,5.基金项目
国家自然科学基金资助项目( No 81202709) （ No 81202709）
浙江省自然科学基金资助项目(No Y207728,LY14H290003) （No Y207728,LY14H290003）
