中国药理学通报Issue(2)：186-193,8.DOI:10.3969/j.issn.1001-1978.2015.02.009
新疆家蚕抗菌肽诱导人胃癌细胞AGS凋亡的研究
Apoptosis of human gastric carcinoma AGS cells induced by cecropinXJ
摘要
Abstract
Aim To investigate the apoptosis of human gastric carcinoma AGS cells induced by cecropinXJ. Methods Human gastric carcinoma AGS cells and human normal epithelial cells GES-1 were co-cultured with different concentrations of cecropinXJ ranging from 0. 01 to 1 000 mg·L-1 for 24 h. MTT assay was used to observe the effects of cecropinXJ on the proliferation of AGS cells and GES-1 cells. The ultrastructural changes of the AGS cells were observed by transmission electron microscopy. Hoechst staining was used to de-tect cell apoptosis. The changes of intracellular reac-tive oxygen species ( ROS) and mitochondrial potential were analysed by flow cytometery. The expression of Bax, Bcl-2, caspase-3 and cytochrome C in mRNA level was investigated by qRT-PCR. Western blot was used to determine the protein expression of Bax, Bcl-2, caspase-3 and cytochrome C. Results CecropinXJ significantly suppressed the proliferation of AGS cells in vitro (P<0. 05) in a dose-dependent manner, IC50 =61. 19 mg·L-1 , but had no inhibitive effects on the proliferation of GES-1 cells. After treatment for 24 h, cecropinXJ induced AGS cells nuclear condensation, and increased ROS production, disrupted mitochondri-al integrity. The results of qRT-PCR and Western blot demonstrated cecropinXJ could up-regulate the expres-sion of Bax and down-regulate the expression of Bcl-2 , promote the release of cytochrome C and activate caspase-3. Meanwhile, cecropinXJ promoted caspase-3 activity in a dose-dependent manner, and cell death ratio of AGS cells induced by cecropinXJ was signifi-cantly reduced by caspase-3 and caspase-9 specific in-hibitors treatment. Conclusion CecropinXJ can in-duce apoptosis of AGS cells by downregulating Bcl-2 , upregulating Bax and activating caspase-3 , which may be one of its anti-tumor mechanisms.关键词
新疆家蚕抗菌肽(cecropinXJ)/胃癌/AGS细胞/细胞凋亡/Bcl-2/Bax/caspase-3/抗肿瘤Key words
cecropinXJ/human gastric carcinoma/AGS cells/cell apoptosis/Bcl-2/Bax/caspase-3/an-ti-tumor分类
医药卫生引用本文复制引用
吴艳玲,夏丽洁,张富春..新疆家蚕抗菌肽诱导人胃癌细胞AGS凋亡的研究[J].中国药理学通报,2015,(2):186-193,8.基金项目
新疆维吾尔自治区高技术研究发展计划项目( No 201110101) （ No 201110101）
