中国药理学通报Issue(2)：273-277,278,6.DOI:10.3969/j.issn.1001-1978.2015.02.025
LC-MS/MS法同时测定CUMS大鼠血浆中3种单胺类神经递质
LC-MS/MS method for simultaneous determination of three monoamine neurotransmitters in rat plasma of CUMS
摘要
Abstract
Aim To establish a LC-MS/MS method for determination of 5-HT, NE, DA and observe the con-centration of 5-HT, NE, DA in rat plasma of CUMS. Methods Twenty-two male SD rats were divided into control group and model group. Model group was given 9 kinds chronic unpredictable mild stimulating factors every day. 21 days later, behavior and orbital blood were measured before and after modeling. Using benzo-yl chloride as a pre-column derivatization reagent, three analytes and IS were derivatized before LC-MS/MS detection. Change in three kinds of neurotransmit-ter concentration was measured in rat plasma before and after modeling. Results After modeling, com-pared with control group, the weight of rats in model group was declined significantly ( P<0. 05 ) . Horizon-tal scores, vertical scores and sugar consumption were declined significantly ( P <0. 01 ) . Calibration curves of 5-HT, NE, DA were linear between 1. 47 ~752, 1. 75 ~898 , 2. 05 ~1 053 μg · L-1 and LOQ were 1. 47, 1. 75, 2. 046μg·L-1 ,respectively. The recov-ery of 5-HT, NE, DA from plasma was over than 70%, and RSD of inter-day and intra-day assay was limited in 15%. Compared with control group, the con-centration of 5-HT, NE, DA in rat plasma of model group was declined to ( 3. 99 ± 1. 21 ) , ( 6. 24 ± 1. 94), (6. 07 ± 1. 98) μg·L-1(P <0. 01). Con-clusion After making CUMS model of depression, three kinds of neurotransmitters in rat plasma are de-creased.关键词
LC-MS/MS/CUMS/大鼠/血浆/5-HT/DA/NE/衍生化Key words
LC-MS/MS/CUMS/rats/plasma/5-HT/DA/NE/derivatization分类
医药卫生引用本文复制引用
臧雨馨,孙冰婷,赵文珠,容纳,戴国梁,居文政,谈恒山..LC-MS/MS法同时测定CUMS大鼠血浆中3种单胺类神经递质[J].中国药理学通报,2015,(2):273-277,278,6.基金项目
国家科技部“重大新药创制”科技重大专项( No 2012ZX09303009-002) （ No 2012ZX09303009-002）
江苏省中医药领军人才( No LJ200906) （ No LJ200906）
江苏高校优学科建设工程资助项目(2010) （2010）
