中国药理学通报Issue(3)：334-338,339,6.DOI:10.3969/j.issn.1001-1978.2015.03.009
米索前列醇对APP/PS1转基因小鼠的神经保护作用
Protective effect of misoprostol on neurodegeneration in APP/PS1 transgenic mice
摘要
Abstract
Aim To investigate the protective effects <br> of misoprostol on hippocampal and cortical neuronal <br> injury in APP / PS1 transgenic mice and its possible mechanism. Methods Mice were divided into 4 groups including youth group, elderly group, APP/PS1 group and misoprostol-treated group. Ten 24-month-old APP / PS1 transgenic mice were randomized into two groups as APP / PS1 group and misoprostol-trearted group. Ten 24-month-old wild-type C57 mice were chosen as elderly group. Mice in misoprostol-treated group were administered with misoprostol ( 200μg · kg-1 d-1 , p. o. ) five days a week for 20 weeks. Mice in the other two groups were administered with carboxy-methylcellulose sodium ( 200 μg · kg-1 d-1 , p. o. ) five days a week for 20 weeks. Three days before miso-prostol treatment was stopped, ten 8-week-old wild-type C57 mice were chosen as youth group. The spatial learning and memory function was evaluated by Morris water maze. The hippocampal and cortical neuronal damage was detected by HE staining. SOD activities and MDA contents were measured by biochemistry method. The expression of Aβ in mice hippocampus and cortex was observed using the immunohistochemi- <br> cal method. Results Compared with those of elderly group,the exploring time of mice was lengthened re-markably, the hippocampal and cortical neurons ap-peared obvious nucleus pycnosis and deep dye, the SOD activities were decreased significantly, the MDA contents and the levels of Aβ increased significantly in APP/PS1 group. Compared with those of APP/PS1 group, the exploring time of mice was shortened re-markably, the damage of hippocampal and cortical neurons reduced significantly, the SOD activities in-creased significantly, the MDA contents and the levels of Aβ decreased significantly in the misoprostol-treated group. Conclusion Our research results suggest that misoprostol has a significant neuroprotective effect on brain injury in APP/PS1 transgenic mice. The neuro-protective mechanism of misoprostol might be related to its antioxidative stress in APP/PS1 transgenic mice.关键词
米索前列醇/APP/PS1/海马/皮层/Aβ/EP/氧化应激/神经保护作用Key words
misoprostol/APP/PS1/hippocampus/cortex/Aβ/EP/oxidative stress/neuroprotective effect引用本文复制引用
纪超男,胡馨月,郭远新,齐云,魏玉玲,阳群芳,杨洋,李昱,杨俊卿..米索前列醇对APP/PS1转基因小鼠的神经保护作用[J].中国药理学通报,2015,(3):334-338,339,6.基金项目
国家自然科学基金资助项目(No 81070972) （No 81070972）
