中国药理学通报Issue(3)：381-387,7.DOI:10.3969/j.issn.1001-1978.2015.03.017
五味子提取物对苯并[ a]芘诱导的早孕期大鼠胚胎损伤的保护作用及凋亡机制研究
Effect of Schisandra chinensis extract on benzo[ a] pyrene-induced apoptosis in embryo of rat and its mechanisms
摘要
Abstract
Aim To investigate the role of Schisandra chinesis extract ( SCE ) in benzo [ a ] pyrene ( BaP )-in-duced apoptosis in embryo of rat and its underlying mechanism. Methods 75 SD female rats were ran-domly divided into 5 groups: control group, model group, low dose group, middle dose group and high dose group with 15 rats in each group. After 15 days of gavage, male and female rats were mated in the same cage. On the ninth day of gestation, all the rats were sacrificed. Body of weight and total organ coefficient ( pregnant uterine total coefficient ) were examined. Levels of serum rat β chorionic gonadotropin and pro-gesterone were determined by ELISA Kits. The mRNA levels of Bax, Bcl-2 and caspase-3 in embryos were determined by real time-PCR, and the expressions of Bax, Bcl-2 and caspase-3 were examined by Western blot. Results ①Compared with control group, the weight of pregnant rats, organ coefficient ( pregnant u-terine total coefficient) , levels of serum ratβchorionic <br> gonadotropin and progesterone in BaP group were de-creased significantly, mRNA and protein expression of Bcl-2 decreased dramatically, while mRNA and protein expression of Bax and caspase-3 increased significant-ly. ②After treatment with SCE the weight of pregnant rats, organ coefficient ( pregnant uterine total coeffi-cient) , levels of serum rat β chorionic gonadotropin and progesterone were increased, mRNA and protein expression of Bcl-2 increased significantly compared with BaP group, while mRNA and protein expression of Bax and caspase-3 were decreased. Conclusion BaP exposure before pregnancy can generate embryo toxicity in rats. SCE can inhibit the reproductive damage in-duced by BaP, and this positive effect may be related to the cell apoptosis mechanism.关键词
五味子提取物/苯并 a芘/早孕期/胚胎毒性/凋亡/Bax/Bcl-2/caspase-3Key words
Schisandra chinesis extract/benzo[ a] py-rene/early pregnancy/embryo toxicity/apoptosis/Bax/Bcl-2/caspase-3引用本文复制引用
梁婧,王华,侯海燕,孙旸,陈亚琼..五味子提取物对苯并[ a]芘诱导的早孕期大鼠胚胎损伤的保护作用及凋亡机制研究[J].中国药理学通报,2015,(3):381-387,7.基金项目
国家自然科学基金资助项目( No 81273977) （ No 81273977）
天津市自然科学基金资助项目(No 12JCYBJC16200) （No 12JCYBJC16200）
