中国药理学通报Issue(3)：396-399,400,5.DOI:10.3969/j.issn.1001-1978.2015.03.020
美洛昔康抑制人结肠癌细胞增殖与Wnt/β-catenin信号的关系研究
Anti-proliferative effect of meloxicam and Wnt/β-catenin signal in colon cancer cells
摘要
Abstract
Aim To investigate the inhibitory effect of meloxicam ( Mel ) on human colon cancer LoVo cells and its mechanism. Methods Crystal violet staining assay and flow cytometry assay were performed to de-termine the anti-proliferative effect of Mel. Flow cy-tometry assay and H&E assay were used to evaluate the apoptotic effect of Mel. Luciferase reporter plasmid ISRE-Luc was employed to measure the effect of Mel on the activation of Wnt/β-catenin signaling transduc-tion. Western blot assay was used to detect effect of Mel on the expression level of β-catenin protein. Re-sults Compared with the control group, the inhibitory rate of cell proliferation at 72 h and 100 μmol · L-1 Mel was ( 57. 635 ± 3. 86 )% ( t = 10. 044 , P =0. 001);the cell apoptotic rate at 24 h and 60 μmol· <br> L-1 Mel was ( 33. 6 ± 1. 7 )% ( t = 30. 166 , P =0. 001 );the transcriptional activities of TCF/LEF and Myc/Max at 24 h and 60 μmol · L-1 were ( 40. 05 ± 2. 79 )% ( t = 14. 864 , P = 0. 001 ) and ( 35. 9 ± 2. 38 )% ( t =16. 904 , P =0. 001 ) respectively; the levels of intracellular β-catenin protein at 24 h or 48 h and 40 μmol·L-1 were (71 ± 3. 78)%(t=7. 353,P=0. 003 ) or ( 52. 66 ± 3. 57 )% ( t = 11. 724 , P =0. 001 ) respectively. Conclusion Mel may inhibit proliferation and promote apoptosis of LoVo cells by in-hibiting Wnt/β-catenin pathway signaling transduction and decreasing intracellular β-catenin protein level.关键词
美洛昔康/人结肠癌 LoVo细胞/增殖抑制/凋亡诱导/Wnt /β-catenin信号/β-catenin蛋白Key words
meloxicam/human colon cancer LoVo cells/anti-proliferation/apoptosis/Wnt /β-catenin signal/β-catenin protein引用本文复制引用
周密,何百成,秧茂盛..美洛昔康抑制人结肠癌细胞增殖与Wnt/β-catenin信号的关系研究[J].中国药理学通报,2015,(3):396-399,400,5.基金项目
国家自然科学基金资助项目(No 81071462,81372120) （No 81071462,81372120）
