中国药理学通报Issue(4)：475-481,7.DOI:10.3969/j.issn.1001-1978.2015.04.008
大鼠佐剂性关节炎病程不同阶段T细胞亚群及重要器官的病理学变化
Variation of T-cell subsets in spleen and tissue histopathological changes in rats with adjuvant arthritis
摘要
Abstract
Aim To observe the variation of T-cell subsets in spleen in different courses of disease and tis-sue histopathological changes in rats with adjuvant ar-thritis (AA).Methods The model of rat AA was in-duced by complete Freund’s adjuvant into the right hind metatarsal footpad of 20 male Lewis rats.Arthritis was evaluated by total score,arthritis index and paw swelling number. The percentage of total (CD3 +CD4 +), memory (CD4 + CD44 +), no-sensitizated (CD4 + CD62L +) and Th1 7 (CD4 + IL-1 7 +) on CD4 +T cells in spleen were detected by flow cytometry on days 1 4 and 22 after immuniozation.The his-topathological evaluation of heart,liver,spleen,lung, kidney,ankle joints and mesenteric lymph nodes were examined by hematoxylin and eosin (HE)stain.Re-sults The onset of secondary arthritis appeared on a-bout day 1 0 after immunization,with a peak onset on day 1 9.Systematic arthritis symptoms included front and hind paw swelling and redness (swelling was more apparent in the front rather than hind paws),nodule formation and redness on the ears,connective tissue swelling and redness of the nose and evident nodules and redness on the tail.Total score,arthritis index and the paw swelling numbers were enhanced in AA rats. Pathohistology of ankles joints showed hyperplasia, pannus formation,and inflammatory cells infiltration in AA rats.And pathohistology of heart,liver,spleen, kidney and mesenteric lymph nodes showed inflamma-tory changes.Compared with normal group,there was no change in the percentage of total,memory and no-sensitizated CD4 +T cell in the early stage inflammation but they were significantly enhanced in the peak of in-flammation.Th1 7 cell subsets were significantly en-hanced in both of the early and peak of inflammation. Conclusion These findings suggest that abnormal T cell responses and tissue histopathological changes are important features of AA rats.关键词
佐剂性关节炎/类风湿关节炎/Th17/病理学/T细胞亚群/病程Key words
adjuvant arthritis/rheumatoid arthritis/Th1 7/histopathology/T cell subsets/disease course分类
医药卫生引用本文复制引用
孙晓静,徐澍,贾晓益,魏芳,杨雪枝,常艳,魏伟..大鼠佐剂性关节炎病程不同阶段T细胞亚群及重要器官的病理学变化[J].中国药理学通报,2015,(4):475-481,7.基金项目
国家自然科学基金资助项目（No 81330081，81173075，31200675）；安徽省科技攻关项目 （）
