中国药理学通报Issue(4)：549-554,6.DOI:10.3969/j.issn.1001-1978.2015.04.022
Salinomycin对乳腺癌阿霉素耐药细胞株MCF-7／DOX的增殖抑制作用及机制
Anti-proliferative effect of salinomycin on doxorubicin-resistant human breast cancer MCF-7/DOX cells
摘要
Abstract
Aim To investigate the anti-proliferative effect of salinomycin on doxorubicin-resistant human breast cancer MCF-7 /DOX cells.Methods MCF-7 and MCF-7 /DOX cells were treated or untreated with salinomycin.Cell viability was detected by MTS assay. Cell apoptosis was detected by Annexin V-FITC /PI as-say.Reactive oxygen species (ROS)was measured by DCFH-DA staining.Mitochondrial membrane potential was measured by JC-1 assay.The expression of apopto-sis related proteins BAX, BCL-2, caspase-3, and caspase-9 were evaluated by Western blot analysis. Results The cell viability was significantly reduced by salinomycin treatment in a dose-dependent manner. The flow cytometry results showed that salinomycin in-duced MCF-7 /DOX cell apoptosis,increased ROS pro-duction,and decreased mitochondrial membrane poten-tial.Furthermore,salinomycin decreased the expres-sion of BCL-2,and increased the expression of BAX, cleaved caspase-3,and cleaved caspase-9.Moreover, the antioxidant N-acetylcysteine (NAC ) markedly blocked the above effects.Conclusions Our results suggest that salinomycin-induced apoptosis in MCF-7 /DOX is associated with induction of ROS production, and activation of mitochondria apoptosis pathway, which may become a potential chemotherapeutic agent for the therapy of doxorubicin resistant breast cancer.关键词
乳腺癌/Salinomycin/阿霉素耐药/细胞凋亡/活性Key words
breast cancer/salinomycin/doxorubicin resistance/cell apoptosis/ROS/mitochondrial mem-brane potential分类
医药卫生引用本文复制引用
刘浩,卢敏莹,贺智敏..Salinomycin对乳腺癌阿霉素耐药细胞株MCF-7／DOX的增殖抑制作用及机制[J].中国药理学通报,2015,(4):549-554,6.基金项目
国家自然科学基金资助项目 （）
