中国药理学通报Issue(4)：582-585,4.DOI:10.3969/j.issn.1001-1978.2015.04.028
固相萃取－高效液相色谱法同时测定血浆中氯米帕明和4种苯二氮艹卓类药物浓度方法的研究
Simultaneous determination of clomipramine and four benzodiazepines in human plasma by HPLC-DAD after solid phase extraction
摘要
Abstract
Aim To establish a novel,highly sensitive,rapid and cost-effective HPLC method to simultaneously determine tri-cyclic antidepressant clomipramine and four benzodiazepines of diazepam,alprazolam,clonazepam,oxazepam in human plasma pretreated by solid phase extraction.Methods The assay was achieved by using C8 column (4.6 mm ×1 50 mm,5 μm)kept at 45 ℃,mobile phase 73.2:26.8 V/V (50 mmol·L -1 ,pH 3.0 phosphate buffer:acetonitrile)with flow rate of 1 .2 ml· min -1 ,and UV detection was set at λ220 nm.Solid phase ex-traction was performed on C1 cartridges.Results The calibra-tion curve was demonstrated to be linear (r >0.9994)in the ranges of 5 ~200 μg·L -1 for alprazolam and clonazepam,1 0 ~500 μg·L -1 for diazepam,20 ~500 μg· L -1 for clomipra-mine,and 7.5 ~2000 μg·L -1 for oxazepam;the limit of detec-tion (LOD)was 1 .5,1 .4,3.0,5.5 and 2.2 μg·L -1 for al-prazolam,clonazepam,diazepam,clomipramine and oxazepam respectively.Intra-day and inter-day precision revealed a coeffi-cient of variation of 2.2% ~1 2.6% and 2.1 % ~1 3.2%,re-spectively.Extraction yield ranged from 81 .1 % ~1 00.1 % for all analytes.Conclusion The developed method is accurate, reproducible,convenient,and suitable for routine therapeutic drug monitoring of clomipramine and the four benzodiazepines.关键词
固相萃取法/氯米帕明/地西泮/奥沙西泮/氯硝西泮/阿普唑仑/高效液相色谱法Key words
solid phase extraction/clomipramine/diazepam/oxazepam/clonazepam/alprazolam/HPLC分类
医药卫生引用本文复制引用
郑付春,石刚刚..固相萃取－高效液相色谱法同时测定血浆中氯米帕明和4种苯二氮艹卓类药物浓度方法的研究[J].中国药理学通报,2015,(4):582-585,4.基金项目
中央财政支持地方高校发展专项资金；广东省自然科学基金团队项目（No 9351503102000001）；广东省自筹经费类科技计划项目 （）
