中国药理学通报Issue(6)：795-800,6.DOI:10.3969/j.issn.1001-1978.2015.06.012
小檗碱对糖尿病肾病大鼠肾组织VEGF表达的影响
Effect of berberine on expression of vascular endothelial growth factor in diabetic nephropathy rats
摘要
Abstract
Aim To investigate the renoprotective effect of berberine in diabetic nephropathy rat model. Methods The rat model of DN was induced by intra-peritoneal injection of streptozotocin ( STZ ) after fed with high sugar and high fat diet for six weeks. The rats were divided into 5 groups randomly, i. e. normal control group, model group, BBR ( 50 mg · kg-1 ) , BBR ( 100 mg · kg-1 ) and BBR ( 200 mg · kg-1 ) treatment group. The fasting blood glucose ( FBG) was evaluated at 2, 4, 6,8 week respectively. The patho-logical changes in the kidney were determined by PAS staining. The expression of vascular endothelial growth factor ( VEGF) was detected by immunohistochemistry and Western blot respectively. Results Compared with normal control group, the value of FBG, SCr, BUN and UTP of model group were sharply increased. <br> Compared with model group, the value of FBG in ber-berine different dosage treatment groups were signifi-cantly decreased to various degrees, and berberine dif-ferent dosage treatment could decrease the levels of SCr, BUN and UTP in different degree. Berberine could surpress the alterations of pathological changes in the kidneys and downregulate the expression levels of VEGF in the kidney of diabetic rats with nephropathy. Conclusion Berberine could significantly ameliorate the biochemical indicators and renal injury of the model rats through affecting the abnormal expression levels of VEGF in the kidney.关键词
小檗碱/糖尿病肾病/血管内皮生长因子/肾小球/足细胞/肾脏保护作用Key words
berberine/diabetic nephropathy/vascu-lar endothelial growth factor/glomerulus/podocyte/renoprotective effect分类
医药卫生引用本文复制引用
倪伟建,丁海华,唐丽琴,魏伟..小檗碱对糖尿病肾病大鼠肾组织VEGF表达的影响[J].中国药理学通报,2015,(6):795-800,6.基金项目
国家自然科学基金资助项目(No 81102864,81073109) （No 81102864,81073109）
安徽省中医药科研课题(No 2012zy48) （No 2012zy48）
