中国药理学通报Issue(6)：815-820,821,7.DOI:10.3969/j.issn.1001-1978.2015.06.016
小补心汤总黄酮对获得性无助小鼠的抗抑郁作用及其对HPA轴功能的影响
Total flavonoids extracted from xiaobuxin-tang on hyperactivity of hypothalamic-pituitary-adrenal axis in mouse learned helplessness model
摘要
Abstract
Aim To investigate the effect of the total flaconoids extracted from Xiaobuxin-Tan g ( XBXT-2 ) <br> on the hyperactivity of hypothalamic-pituitary-adrenal axis in mouse learned helplessness model. Methods Learned helplessness was induced by inescapable foot shock stress over 1h session for 5 days. After screen-ing, we divided learned helplessness mice into five groups:IS, inescapable shock;Dlx, dulxetine(20 mg ·kg-1);XBXT-2(25,50 mg·kg-1). Latency to es-cape shocks and escape failure had been recorded. During the test, Dlx(20 mg·kg-1 ) and XBXT-2(25, 50 mg·kg-1 ) were administered intragastrically once daily for four days. Serum corticosterone level and ad-renocorticotropic hormone ( ACTH ) level were meas-ured by ELISA, and expression of glucocorticoids re-ceptor ( GR) α/β and brain-derived neurotrophic fac-tor ( BDNF ) in hippocampus was determined using Western blot method. Results XBXT-2 (25,50 mg· <br> kg-1 ) or duloxetine treatment showed antidepressant effect in mouse learned helplessness model, as demon-strated by the decreased escape failure and escape la-tency. Our ELISA results showed that XBXT-2 or du-loxetine significantly decreased serum corticosterone level and its upstream stress hormone ACTH level in learned helplessness mice. Furthermore, Western blot result demonstrated XBXT-2 treatment increased GRs and BDNF expression in hippocampus. Conclusions XBXT-2 produces significant antidepressant effect on learned helplessness mice. In addition, the modulation of HPA axis produced by XBXT-2 may be important mechanism underlying its antidepressant-like effect in mouse learned helplessness model.关键词
小补心汤总黄酮/抗抑郁/获得性无助模型/行为/HPA轴/脑源性神经营养因子Key words
XBXT-2/antidepressant effect/learned helplessness model/behavior/HPA axis/BDNF分类
医药卫生引用本文复制引用
陈力,张有志,薛瑞,于能江,王玉露,胡晓旭,仇志坤,赵楠,李云峰,徐江平..小补心汤总黄酮对获得性无助小鼠的抗抑郁作用及其对HPA轴功能的影响[J].中国药理学通报,2015,(6):815-820,821,7.基金项目
国家科技重大专项新药创制专项( No 2012ZX09102101-004) （ No 2012ZX09102101-004）
国家自然科学基金资助项目( No 81302761,81274117,30973516) （ No 81302761,81274117,30973516）
