中国药理学通报Issue(6)：827-832,833,7.DOI:10.3969/j.issn.1001-1978.2015.06.018
mmLDL对小鼠肠系膜动脉α1受体介导的血管收缩及相关蛋白表达影响的研究
Study of α1 adrenoceptor-mediated vasoconstriction and protein expressions induced by mmLDL in mouse mesenteric artery
摘要
Abstract
Aim To investigate the effects of mmLDL on the up-regulation ofα1 receptors in moues mesenter- <br> ic arteries. Methods Mice tail intravenous injection of mmLDL was used . Vitro sensitive myograph was empl- <br> oyed to examine Noradrenaline ( NA) induced vascular contraction on mice mesenteric artery, and the mRNA and protein expressions ofα1 andα2 receptors were an-alyzed by real-time PCR and Western blot, respective-ly. Results mmLDL significantly increased NA in-duced concentration-contractile curve, and the data of Emax and pEC50 were from ( 122. 61 ± 9. 40 )% and (5. 65 ± 0. 05 ) in normal saline ( NS ) group to (161. 01 ± 6. 90 )% and ( 6. 20 ± 0. 08 ) in mmLDL group (P <0. 01, P <0. 01), respectively. The α1 adrenoceptor antagonist prazosin shifted the concentra- <br> tion-contractile curve induced by NA towards right. Af-ter using mmLDL, the mRNA and protein levels of α1 adrenoceptor were significantly increased, but the mR-NA and protein levels of α2 adrenoceptor were not changed. Conclusion Tail intravenous injection of mmLDL enhances the vascular expressions of α1 adre-noceptors and the contractile effects mediated byα1 ad-renoceptors.关键词
mmLDL/肠系膜动脉/α1 受体/哌唑嗪/育亨宾/尾静脉注射Key words
mmLDL/mesenteric artery/α1 adreno-ceptor/prazosin/yohimbine/tail intravenous injection分类
医药卫生引用本文复制引用
郭立军,江高峰,李海鹏,李琼,刘恩岐,李洁..mmLDL对小鼠肠系膜动脉α1受体介导的血管收缩及相关蛋白表达影响的研究[J].中国药理学通报,2015,(6):827-832,833,7.基金项目
国家自然科学基金资助项目( No 81202535) （ No 81202535）
郴州市科技局资助项目(No CZ2014015) （No CZ2014015）
