中国药理学通报Issue(6)：838-843,6.DOI:10.3969/j.issn.1001-1978.2015.06.020
蛇葡萄素钠联合卡铂对人肺腺癌GLC-82细胞增殖及凋亡的影响
Effect of ampelopsin sodium combined with carboplatin on the proliferation and apoptosis of human lung adenocarcinoma cell GLC-82
摘要
Abstract
Aim To investigate the cytotoxic effect and mechanism of ampelopsin sodium ( AMP-Na ) on hu-man lung adenocarcinoma cell line GLC-82 alone or combined with carboplatin ( CBP ) . Methods The cytotoxic effect of human lung adenocarcinoma cell line GLC-82 was investigated by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide ( MTT ) colori-metric assay. Ultrastructure change of apoptotic GLC-82 cells was observed with transmission electron micro-scope. The changes of the cell apoptosis and the ex-pression of caspase-3 were analyzed with flow cytome-ter. Results Combined with AMP-Na, the IC50 of CBP decreased from (17. 10 ± 4. 78) mg·L-1 to <3. 12 mg·L-1(P<0. 01), showed that the combina-tion of AMP-Na and CBP had synergistic effect on GLC-82 cells ( CDI <1 ) . As with transmission elec- <br> tron microscope and flow cytometric analysis, the apop-tosis and necrosis ratios also increased in the combina-tion group. The necrosis ratios increased from (2. 56 ± 0. 41 )% to ( 71. 83 ± 5. 43 )% ( P<0. 01 ) . The ex-pression of caspase-3 was increased significantly after treated with AMP-Na or combined with CBP. Conclu-sions There is a synergistic cytotoxic effect on GLC-82 cells treated with AMP-Na combined with CBP. Ap-optotic cells and necrotic cells are found in GLC-82 cells treated with AMP-Na alone or combined with CBP. One of the mechanisms to induce apoptosis is probably that activation of caspase-3 mediates signal transduction pathway in cells.关键词
蛇葡萄素钠/卡铂/人肺腺癌 GLC-82 细胞/细胞凋亡/细胞坏死/caspase-3Key words
ampelopsin sodium/carboplatin/human lung adenocarcinoma cell line GLC-82/apoptosis/nec-rosis/caspase-3分类
医药卫生引用本文复制引用
王霞,韩伟,葛斌,吴勇杰..蛇葡萄素钠联合卡铂对人肺腺癌GLC-82细胞增殖及凋亡的影响[J].中国药理学通报,2015,(6):838-843,6.基金项目
广东省科技厅省部产学研合作项目( No 2007A090302025) （ No 2007A090302025）
