中国药理学通报Issue(7)：994-998,999,6.DOI:10.3969/j.issn.1001-1978.2015.07.021
银杏二萜内酯葡胺注射液对缺糖缺氧损伤的SH-SY5 Y细胞保护作用
Neuroprotective effects of YXETNZ injection on SH-SY5 Y cells against injury induced by oxygen-glucose deprivation
摘要
Abstract
Aim To investigate the protective effects of YXETNZ injection on SH-SY5 Y cells damaged by oxygen-glucose deprivation ( OGD ) , and explore its functional mechanisms. Methods After 4 h of OGD, the cells were treated with 25 mg·L-1 drugs for 1 h. Subsequently, cell viabilities were measured by cell counting kit-8 ( CCK-8 kit ) and cell apoptosis was measured by caspase-3/7 assay kit according to manu-facturer’ s instructions. Furthermore, cell death was also detected by ELISA. The levels of phospho-Akt, phospho-PKA,phospho-Bad were evaluated by western blot. Results Oxygen-glucose deprivation significant-ly decreased the cell viabilities of SH-SY5Y cells, while YXETNZ injection significantly increased cell vi-abilities, phospho-Akt, phospho-PKA and phospho-Bad. Furthermore, YXETNZ injection also reduced the activities of caspase-3/7 and cytoplasmic histone-asso-ciated-DNA-fragments contents. Conclusion Our re-searches demonstrat that YXETNZ injection shows good neuroprotective effects on SH-SY5 Y cells after oxygen-glucose deprivation. The underlying mechanisms may be associated with activation of PI3 K/Akt/Bad/caspase-3/7 , cAMP/PKA/Bad/caspase-3/7 signaling pathway.关键词
银杏二萜内酯葡胺注射液/缺糖缺氧损伤/细胞凋亡/PKA/Akt/BadKey words
YXETNZ injection/oxygen-glucose dep-rivation/apoptosis/PKA/Akt/Bad分类
医药卫生引用本文复制引用
刘秋,许治良,周军,李娜,毕宇安,王振中,萧伟..银杏二萜内酯葡胺注射液对缺糖缺氧损伤的SH-SY5 Y细胞保护作用[J].中国药理学通报,2015,(7):994-998,999,6.基金项目
现代中药创新集群与数字制药技术平台( No 2013ZX09402203) （ No 2013ZX09402203）
国家工信部重大科技成果转化项目 （）
