中国药理学与毒理学杂志Issue(3)：329-333,5.DOI:10.3867/j.issn.1000-3002.2014.03.003
电刺激大鼠上矢状窦区硬脑膜后血浆与脑组织中降钙素基因相关肽、P物质和前列腺素 E2释放差异
Release difference of calcitonin gene-related peptide,substance P and prostaglandin E2 in orbital venous plexus plasma and brain tissues from rats with dura mater of superior sagittal sinus following electrical stimulation
陈健 1吕新勇 1孙晓萍 1陈春苗 1周军 1王振中 1萧伟1
作者信息
- 1. 江苏康缘药业股份有限公司 中药制药过程新技术国家重点实验室 江苏省企业院士工作站，江苏 连云港 222001
- 折叠
摘要
Abstract
OBJECTIVE Toexplorethefeasibilityofpreparingamigrainemodelusingratdura materofsuperiorsagittalsinusinfollowingelectricalstimulation.METHODS MaleSDratswereexposed to 1 h electrical stimulation after brain electrode implantation,at 3 V,6 Hz,and a pulse of 0.25 ms.At 0, 1 5,30 min,and 1 h,orbital blood and brain tissue were taken.The content of calcitonin gene-related peptide (CGRP),substance P and prostaglandin E2 (PGE2 )in the plas ma and brain tissue was detec-tedbyradioimmunoassay.RESULTS ThebraintissuecontentofCGRP,substancePandPGE2in the sham group and normal group was not significantly different.CGRP,substance P and PGE2 in brain tissue of model group were 3.41 ±0.93,1 .80 ±0.64,3.41 ±0.93 and (1 .80 ±0.64)ng·g -1 respec-tively,significantly different from sham group(P<0.05).At different time points,the content of CGRP, substance P and PGE2 in plasma orbital venous plexus showed no significant difference between sham groupandmodelgroup.CONCLUSION ThereleaseofCGRP,PGE2andsubstancePinbraintissue is significantly different fro m that of plas ma after electrical sti mulation.The results suggest that a model of migraine can be constructed by electrical stimulation.关键词
偏头痛/电刺激/硬脑膜/降钙素基因相关肽/P物质/前列腺素E2Key words
migraine/electrical stimulation/dura mater/calcitonin gene-related peptide/substance P/prostaglandin E2分类
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陈健,吕新勇,孙晓萍,陈春苗,周军,王振中,萧伟..电刺激大鼠上矢状窦区硬脑膜后血浆与脑组织中降钙素基因相关肽、P物质和前列腺素 E2释放差异[J].中国药理学与毒理学杂志,2014,(3):329-333,5.
