中国药理学与毒理学杂志Issue(3)：415-420,6.DOI:10.3867/j.issn.1000-3002.2014.03.018
T-2毒素对小鼠胚胎干细胞线粒体功能的抑制作用
T-2 toxin inhibits mitochondrial function of differentiated murine embryonic stem cells
摘要
Abstract
OBJECTIVE ToexplorethepossiblemechanismoractiontargetsofT-2toxinembryo toxicity by observing the effect of T-2 toxin on mitochondrial function of differentiated murine e mbryonic stemcells(mESCs).METHODS Duringdifferentiationat24,72and120h,ESCswereexposedto T-2 toxin 0.5 μg·L-1 .Meanwhile,mESCs were pre-treated with antioxidant Trolox (200 μmol·L-1 )for 30 min and exposed to T-2 toxin (0.5 μg·L-1 )for 72 h.The mitochondrial ultrasture of differentiated mESCs was observed under a transi mission electrical microscope (TEM).The differentiated ESC mito-chondrial function,including respiratory control ratio (RCR),ATP synthase activity and mitochondrial membranepotential(MMP),wasmeasuredat144hafterdifferentiation.RESULTS Significant decrease of the mitochondrial number,deformation of mitochondrial structure,and lack of complete mito-chodrial crest were observed through TEM in the groups of T-2 toxin exposed for 72 and 1 20 h,respec-tively.Compared with the normal control group,RCR declined by 49.5% and 55.1%,ATP synthase activity decreased by 84.9% and 89.3%,and MMP decreased by 23.2% and 35.2% in T-2 toxin 0.5 μg·L-1 exposure 72 and 1 20 h group,respectively.However,the inhibition of mitochondrial function by T-2 toxin in differentiated mESCs recovered significantly in the presence of the antioxidant Trolox. CONCLUSION T-2toxininducesoxidativestressandinhibitsmESCsmitochondrialfunctionindifferenti-ated mESCs,and ROS-induced mitochondrial malfunction plays an i mportant role in T-2 toxin e mbryonic toxicity mechanis m.关键词
T-2毒素/毒性作用/胚胎干细胞/线粒体/细胞分化Key words
T-2 toxin/toxic actions/e mbryonic ste m cells/mitochondrial/cell differentiation分类
医药卫生引用本文复制引用
方海琴,李利忠,赵增明,何俊,赵君,杨嵘,耿雪,彭双清..T-2毒素对小鼠胚胎干细胞线粒体功能的抑制作用[J].中国药理学与毒理学杂志,2014,(3):415-420,6.基金项目
The project supported by International Cooperation Project from Ministry of Science and Technology of China （2011 DFA32190）；National Natural Science Foundation of China （81172699）；National Natural Science Foun-dation of China （81302462）；and Natural Science Foundation of Beijing City （7142128）基金项目国家科技部国际合作项目（2011 D FA32190）；国家自然科学基金项目（81172699）；国家自然科学基金项目（81302462）；北京市自然科学基金资助项目 （）
