中国药理学与毒理学杂志Issue(4)：519-524,6.DOI:10.3867/j.issn.1000-3002.2014.04.008
牵拉对毒蕈碱受体和5-羟色胺受体介导大鼠离体胃平滑肌收缩功能的影响
Effect of stretch on muscarinic receptor- and 5-hydroxytryptamine receptor-mediated contractile responses in isolated gastric smooth muscle of rats
摘要
Abstract
OBJECTlVE To investigate the effect of stretch on muscarinic receptor- and 5-hydroxytryptamine( 5-HT)receptor-mediated contractiIe responses in isoIated circuIar muscIe strips taken from the rat stomach. METHODS The contractiIe responses to carbachoI(CCh)0.001-30 μmoI·L-1 or 5-HT 0.0001 -30 μmoI·L-1 administered cumuIativeIy were recorded in isoIated circuIar muscIe strips taken from the gastric fundus,body,cardia and pyIorus of rats under different preIoads of 1.0,1.5,2.0, 2.5 and 3. 0 g,but a singIe concentration of CCh 0. 3 μmoI·L-1 was administered to the antrum. RESULTS The -Log EC50 vaIue for CCh in the isoIated circuIar muscIe strips of the gastric fundus, body and pyIorus under 1.0 g preIoad was the Iargest,but decreased significantIy with higher preIoads (P﹤0.05,P﹤0.01). A simiIar resuIt was obtained in the isoIated circuIar muscIe strips of the gastric body when 5-HT was used as an agonist. The Emax vaIue of contractiIe responses to CCh and 5-HT in the cir-cuIar muscIe strips of the gastric cardia under 3.0 g preIoad was increased by 117.4% and 75.7% com-pared to that under 1.0 g preIoad. The Emax vaIue of contractiIe responses to 5-HT in the circuIar muscIe strips of the gastric body under 3.0 g preIoad was aIso increased by 115.9% when compared to 1.0 g preIoad. The Emax( g) vaIue of contractiIe responses to CCh in four types of muscIe strips was 10.453±2.956(cardia under 3.0 g preIoad),13.878±2.618(fundus under 2.5 g preIoad),10.244±1.843 (gastric body under 2.5 g preIoad)and 2.585±1.098(pyIorus under 2.5 g preIoad),respectiveIy. The Emax(g)vaIue of contractiIe responses to 5-HT in three types of muscIe strips was 4.363±1.705(cardia under 3.0 g preIoad),3.931±0.615(fundus under 3.0 g preIoad)and 3.161±0.680(gastric body under 3.0 g preIoad),respectiveIy. CONCLUSlON 0.5 g or 1.0 g preIoad is inadequate for accurate determi-nation of contractiIe responses mediated by muscarinic receptors and 5-HT receptors in isoIated circuIar muscIe strips taken from the rat gastric cardia,fundus,body and pyIorus,but 2.0 g preIoad is optimaI for investigating muscarine receptor- or 5-HT receptor-mediated contractiIe responses of isoIated gastric strips of rats.关键词
受体,毒蕈碱/受体,5-羟色胺/牵拉/胃体/胃底/贲门/幽门Key words
receptors,muscarine/receptors,5-hydroxytryptamine/stretch/gastric body/fundus/cardia/pyIorus分类
医药卫生引用本文复制引用
王东凯,任雪姣,卢丹丹,任雷鸣..牵拉对毒蕈碱受体和5-羟色胺受体介导大鼠离体胃平滑肌收缩功能的影响[J].中国药理学与毒理学杂志,2014,(4):519-524,6.基金项目
国家重点基础研究发展计划（973计划）（2012 CB518601）；国家科技重大专项（2011ZX09102-011-04）@@@@The project supported by NationaI Program on Key Basic Research Project of China（973 Program）（2012CB518601）；and NationaI Science & TechnoIogy major Project of China （）
