中国药理学与毒理学杂志Issue(2)：213-219,7.DOI:10.3867/j.issn.1000-3002.2015.02.005
连花清瘟胶囊对脂多糖致急性肺损伤小鼠炎症因子和连接蛋白表达的影响
Effect of Lianhuaqingwen capsules on inflammatory cytokines and junction protein expression in mice with acute lung injury induced by lipopolysaccharides
摘要
Abstract
OBJECTIVE To explore the effect of Lianhuaqingwen capsules ( LHQW ) on junction protein expression in mouse lung tissue of lipopolysaccharide (LPS)-induced acute lung injury ( ALl). METHODS 120 male mice were randomly divided into six groups: normal control, model, model+dexa-methasone 5 mg.kg-1 , model +LHQW 2, 4 and 8 g.kg-1 groups. Dexamethasone and LHQW were administered orally, once daily, for 7 d. 24 h after the last administration, LPS solution was instilled into the tracheas of mice except the normal control group to prepare the mouse model of ALl. 24 h after the establishment of the ALl model, the mice were sacrificed and the pathological changes in the mouse lung tissue were observed by optical microscopy and ultrastructure of alveolar epithelium was observed by transmission electron microscopy. The cell percentage of positive expression of tumor necrosis factor-α(TNF-α) in the peripheral blood T lymphocytes was detected by flow cytometry. The expressions of con-nexin 43 ( Cx43), occludin and zonula occludens protein-1 ( ZO-1) in lung tissues were detected by immunohistochemistry. RESULTS Under the light microscope, the mouse lung of model group showed a large amount of inflammatory cell infiltration and alveolar wall thickening. Compared with model group, inflammatory cell infiltration was reduced in model+dexamethasone, model+LHQW 2,4 and 8 mg.kg-1 groups. Under the electron microscope, the mouse alveolar epithelial cells of model group showed injury. Compared with model group, the damage was reduced in model+dexamethasone, and model+LHQW 2, 4 and 8 mg.kg-1 groups. The cell percentage of TNF-α positive expression in peripheral blood T lympho-cytes in normal control, model, model+dexamethasone, model+LHQW 2,4 and 8 mg.kg-1 groups was (3.6±0.9)%, (6.4±0.8)%, (2.8±0.7)%, (4.7±1.6)%, (4.0±1.5)% and (3.6±1.2)%, respectively. The percentage in model group was obviously higher than that in normal control group( P<0.01), but was lower in the four drug treatment groups than in model group(P<0.05, P<0.01). The expression of Cx43, occludin and ZO-1 in lung tissue of model group was lower than that of normal control group(P<0.01), but higher in model+dexamethasone, model + LHQW 4 and 8 mg.kg-1 groups than in model group(P<0.05). CONCLUSION LHQW may alleviate ALl induced by LPS and play a protective role by inhibiting inflammatory cell infiltration and improving protein connection expression in alveolar epithelial cells and pulmonary vascular endothelial cells.关键词
连花清瘟胶囊/脂多糖/急性肺损伤/连接蛋白Key words
Lianhuaqingwen capsules/lipopolysaccharides/acute lung injury/junction protein分类
医药卫生引用本文复制引用
崔雯雯,金鑫,张彦芬,王宏涛,秘尧,何奇龙..连花清瘟胶囊对脂多糖致急性肺损伤小鼠炎症因子和连接蛋白表达的影响[J].中国药理学与毒理学杂志,2015,(2):213-219,7.基金项目
国家科技重大专项(2011ZX09201-201-27)河北省中医药管理局科研计划(2014227)@@@@The project supported by National Science and Technology Major Project of Original New Drug Research of China(2011ZX09201-201-27)and Scientific Research Program of Hebei Province Administration of Traditional Chinese Medicine(2014227) （2011ZX09201-201-27）
