中国药物评价Issue(1)：6-7,16,3.
HPLC法检测对氯苯甲酰氯中位置异构体杂质
Determination of Position Isomers in Parachlorobenzoyl Chloride by HPLC
陈凤琴 1宋雪洁 1石莹 1张贤斌 1孙春艳1
作者信息
- 1. 扬子江药业集团有限公司药物研究院，江苏泰州225321
- 折叠
摘要
Abstract
Objective:HPLC method has been developed for sensitive determination of 2-chlorobenzoyl chloride and 3-chlorobenzoyl chloride ( position isomers) in parachlorobenzoyl chloride.Methods: After adding water complete hydrolysis of parachlorobenzoyl chlo-ride samples, 2-chlorobenzoyl chloride and 3-chlorobenzoyl chloride were separated and analyzed on a SUPELCO Discovery C-18 column (250 mm ×4.6 mm,5μm) with acetonitrile /phosphoric acid water solution (pH=2.5) as the mobile phase at a flow rate of 1.0 mL · min-1 , the detection wavelength was 230nm and the column temperature was 25 ℃.Results:The results showed that the linear ranges were from 1.55 to 9.31 ×10 -6 mol· L-1 for 2-chlorobenzoyl chloride and 3-chlorobenzoyl chloride by the external standard method.The linear regression equations were Y2 =4.6977X-0.3132(r=0.9991),Y3 =6.0618X+0.9108(r=0.9992),respectively.The detection limits of 2-chlorobenzoyl chloride and 3-chlorobenzoyl chloride were 1.14 ×10 -7 mol· L-1 and 0.74 ×10-7 mol· L-1 ,respectively.The average recoveries of 2-chlorobenzoyl chloride and 3-chlorobenzoyl chloride were 95.8% and 98.7% with the relative standard devia-tions of 1.23%and 1.86%,respectively.Conclusion:The method is accurate,reliable,sensitive,simple and suitable for the determina-tion of position isomers in parachlorobenzoyl chloride.关键词
对氯苯甲酰氯/异构体/高效液相色谱Key words
Parachlorobenzoyl chloride/Position isomers/High performance liquid chromatography( HPLC)分类
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陈凤琴,宋雪洁,石莹,张贤斌,孙春艳..HPLC法检测对氯苯甲酰氯中位置异构体杂质[J].中国药物评价,2015,(1):6-7,16,3.
