中国医学创新Issue(18)：69-71,3.DOI:10.3969/j.issn.1674-4985.2014.18.023
乏氧条件下miR-29a通过AMPK调控胰岛β细胞功能的研究
Study on the Function of AMPK Regulation in PancreaticβCells under Hypoxic Conditions Via miR-29a
王秀娟 1孙尧2
作者信息
- 1. 河北联合大学 河北 唐山 063000
- 2. 河北联合大学附属医院
- 折叠
摘要
Abstract
To explore the mechanism by which miRNA plays roles in pancreatic β cells through regulating AMPK under hypoxic condition. Method:Under the condition of lack of oxygen miR-29 a by AMPK regulation islet beta cell function were observed by cell culture and Real-time PCR.Result:The INS-1 cell primarily expressed AMPKα2,which the phosphorylation activity of AMPK was increased under hypoxic condition. the expression of AMPK was detected by Real time PCR,which suggested that pancreaticβcells primarily expressed AMPKα2 under hypoxic condition. Hypoxia could accelerate the expression of HIF-1αand the activation of AMPK. Meanwhile,AMPK might be regulated by miR-29a.Conclusion:Under the condition of lack of oxygen islet beta cells mainly expressed as AMPKa2, AMPK can promote cell proliferation islet beta cells.关键词
AMPK/胰腺β细胞/乏氧/miR-29aKey words
AMPK/Pancreaticβcells/Hypoxia/miR-29a引用本文复制引用
王秀娟,孙尧..乏氧条件下miR-29a通过AMPK调控胰岛β细胞功能的研究[J].中国医学创新,2014,(18):69-71,3.
