中国医学创新Issue(21)：1-5,6,6.DOI:10.3969/j.issn.1674-4985.2014.21.001
结直肠癌有无肝转移血清差异蛋白质表达的研究
A Research Study of the Serum Proteomics Expressions on Liver Metastases Value in Colorectal Carcinoma
摘要
Abstract
To use proteomics separation method identify colorectal carcinoma patients’ serum proteins,so as to differentiate and diagnostic the serum protein biomarkers for liver metastases.Method:According to inclusion criterion,patients had been divided into colorectal cancer patients with liver metastases,and patients without liver metastases. Two groups were be randomized into 12 serum samples,same amount of serum proteins were mixed in each group by two-way clear gel electrophoresis,followed by building two sets of two-dimensional electrophoresis pattern. Image-Master V5.0 software was used to find the differences between two samples. Kam proteins were differentiated by Matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS). The differentiated expressed proteins in given bioinformatics databases was analyzed.Result:The colorectal liver metastases group and the non-colorectal liver metastases group showed that eight proteome were spotted with more than two differentials,of which five proteins were up-regulated and two were down-regulated. Each protein gray body average differences between the two groups had statistical significance(P<0.05). Five up-regulated proteins were:Transferrin,Complement component C9, RNA directed DNA polymerase,Conserved hypothetical-protein,SEC14L1 7 kDa protein;two down-regulated proteins were Haptoglobin and Serum albumin Isoform 1.Conclusion:Serum protein expression spectrum shows differential in colorectal carcinoma patients with liver metastases. This differential may be the diagnostic serum biomarkers for liver metastases.关键词
结直肠癌/肝转移/差异蛋白质Key words
Colorectal Carcinoma/Liver Metastasis/Proteomics引用本文复制引用
张伟斌,陈丹,马伟钦,何兴祥,陈羽,王丽京..结直肠癌有无肝转移血清差异蛋白质表达的研究[J].中国医学创新,2014,(21):1-5,6,6.基金项目
国家自然科学基金（61201437）；广东省医学科研基金项目（A2011315）；广东省高等学校高层次人才项目 （）
