中国医学创新Issue(30)：23-26,4.DOI:10.3969/j.issn.1674-4985.2014.30.008
在无创性通气条件下快速建立小鼠心肌梗死模型的简易方法
Efficient Establishment of Mice Myocardial Infarction Model in Non-invasive Ventilation
卢新林 1岳玉霞 2严泉祥 1何原 3陈文江 1陈灿3
作者信息
- 1. 广东医学院 广东 湛江 524023
- 2. 河南大学附属淮河医院
- 3. 广东医学院附属医院
- 折叠
摘要
Abstract
Objective:To discuss the method of establishing an efficient mice myocardial infarction model with a high survival rate.Method:The mice were anesthetized with pentobarbital sodium,thoracotomy in non-invasive ventilation,gently squeeze out the heart of the chest,off the hook of the left anterior descending coronary artery with operation needle,by observing the survival rate,the change of electrocardiogram,used pathology testing to prove the model to be successful. At the same time compared with the coronary artery ligation group.Result:After off the hook of the left anterior descending coronary artery,the ST-T segment of ECG raised,the infarction area was obvious with TTC staining,myocardial fiberizes 4 weeks after operation;the survival rate of myocardial infarction model was 85%,the ligation of the coronary artery group was 54%.Conclusion:Application of this new method can establish an efficient mice myocardial infarction mode.关键词
心肌梗死/戊巴比妥钠/机械通气/冠状动脉结扎Key words
Myocardial infarction/Pentobarbital sodium/Non-invasive ventilation/Ligation of the coronary artery引用本文复制引用
卢新林,岳玉霞,严泉祥,何原,陈文江,陈灿..在无创性通气条件下快速建立小鼠心肌梗死模型的简易方法[J].中国医学创新,2014,(30):23-26,4.
