中国医学创新Issue(4)：14-17,4.DOI:10.3969/j.issn.1674-4985.2015.04.005
NF-κB抑制剂Bay 11-7082对人胰腺癌细胞株SW1990增殖和凋亡的影响
The Effect of NF-κB Inhibitor Bay 11-7082 on Proliferation and Apoptosis in Human Pancreatic Cancer ;Cell Line SW1990
张三军1
作者信息
- 1. 湖南省岳阳市第一人民医院 湖南 岳阳 414000
- 折叠
摘要
Abstract
Objective:To study the effect of blocking the NF-κB signaling pathway on proliferation and apoptosis in human pancreatic cancer cell line SW1990 and its possible mechanism.Method:SW1990 cells were treated with different concentrations of NF-κB inhibitor Bay 11-7082(2.5μmol/L,5μmol/L 和 10μmol/L),SW1990 cells morphological changes after treatment were observed under the optical microscope and fluorescence microscopy.CCK-8 assay was used to evaluate the growth influence of Bay 11-7082 on pancreatic cancer SW1990 cells,the flow cytometry was used to determine apoptosis in SW1990 cells.Western blot was used to detect the protein expression of caspase-3 and Bax.Result:A dose-dependent inhibitory effect of Bay 11-7082 on pancreatic cancer SW1990 cells was observed.The result of FCM indicated that treated with Bay 11-7082 exhibited a significant increase of apoptosis in a dose-dependent manner.The expression of caspase-3 and Bax was up-regulated in human pancreatic cancer SW1990 cells after treatment. Conclusion:Bay 11-7082 inhibits the proliferation of SW1990 cells,which is mediated by altering apoptosis-related genes through up-regulation of caspase-3 and Bax.关键词
胰腺肿瘤/凋亡/NF-κBKey words
Pancreatic neoplasm/Apoptosis/NF-κB引用本文复制引用
张三军..NF-κB抑制剂Bay 11-7082对人胰腺癌细胞株SW1990增殖和凋亡的影响[J].中国医学创新,2015,(4):14-17,4.
