中国医学创新Issue(6)：1-4,4.DOI:10.3969/j.issn.1674-4985.2015.06.001
miR-103a-3p在胰腺癌细胞中的表达及意义
Expression and Significance of miR-103a-3p in Pancreatic Carcinoma
摘要
Abstract
Objective: To observe the expression inhibition of miR-103-a-3p in PANC-1 pancreatic cancer cell lines infected by lentivirus with miR-103a-3p-inhibitor, and to provide theoretical basis for elucidating the pathogenesis of pancreatic cancer and screening novel target genes for the treatment of .pancreatic cancer.Method:The expression of miR-103a-3p in two pancreatic cancer cell strains (PANC-1 and BxPC-3) with different invasiveness and the normal pancreatic ductal epithelial cell line (H6C7) were detected by using qRT-PCR. Lentivirus vectors with miR-103a-3p-inhibitor were constructed and used to infect PANC-1 cell with high expression level of miR-103a-3p. Transfection efficiency was observed by fluorescence microscope and the expression of miR-103a-3p were detected by qRT-PCR. Result:qRT-PCR showed that the relative quantitative expressions of miR-103a-3p in PANC-1, BxPC-3 and H6C7 cell strains were (4.949±0.130), (1.417±0.120) and (1.010±0.151), respectively. miR-103a-3p was expressed at highest level in PANC-1 cell. After infected by lentivirus with miR-103a-3p-inhibitor, PANC-1 cells were found to express green fluorescent proteins at high level. qRT-PCR indicated that there were no significant differences in the relative quantitative expressions of miR-103a-3p between PANC-1 cells uninfected by lentivirus with miR-103a-3p-inhibitor vector (1.002±0.160) and PANC-1 cells infected by lentivirus with negative control vector (0.956±0.250), but significant reduction of the relative quantitative expressions of miR-103a-3p in PANC-1 cells infected by lentivirus with miR-103a-3p-inhibitor vector (0.317±0.320) were detected(P<0.05).Conclusion:miR-103a-3p is expressed at higher level in pancreatic cancer cells with stronger invasion ability. miR-103a-3p-inhibitor can be transfected stablely into PANC-1 cells and inhibit the expression of miR-103a-3p.关键词
miR-103a-3p/胰腺癌/慢病毒Key words
miR-103a-3p/Pancreatic cancer/Lentivirus引用本文复制引用
贺军,曹金敏,陈国栋,贺更生,凌晖..miR-103a-3p在胰腺癌细胞中的表达及意义[J].中国医学创新,2015,(6):1-4,4.基金项目
国家自然科学基金（81071966）；湖南省科技厅项目 （）
