中国医学创新Issue(10)：18-20,3.DOI:10.3969/j.issn.1674-4985.2015.10.006
Fas及Fas-L对小梁切除术后成纤维细胞的凋亡研究
Research on Apoptosis of the Fibroblast Induced by Fas and Fas-L after Trabeculectomy
摘要
Abstract
Objective: To explore apoptosis of the fibroblast induced by Fas and Fas-L after trabeculectomy. Method: A total of 20 healthy white rabbits were randomly divided into the control group and observation group,10 cases in each group,and the rabbits in the observation group were given glaucoma trabeculectomy.Two groups of tissues in trabeculectomy filtration area, which contain conjunctiva, anadesma, sclera and limbus corneae were cut down, and the fibroblasts of the tissues were cultivated. 5 cases of fibroblasts in each group were randomly selected and treated by FasMcAb. At last, PI staining and flow cytometry were used for testing the apoptotic rate of all the fibroblasts.Result:The apoptotic rate of normal fibroblasts induced by FasMcAb was higher than that of normal fibroblasts without induced by FasMcAb, the difference was statistically significant(P<0.01); the apoptotic rate of fibroblasts from tissues in trabeculectomy filtration area induced by FasMcAb was higher than that of fibroblasts from tissues in trabeculectomy filtration area without induced by FasMcAb,the difference was statistically significant(P<0.01);the effect of FasMcAb on the apoptotic rate of fibroblasts from tissues in trabeculectomy filtration area was more significant than that on normal fibroblasts, the difference was statistically significant(P<0.01).Conclusion:Fas/Fas-L can accelerate apoptosis of the fibroblast after trabeculectomy, and helps to find a new breakthrough to reduce the scar after glaucoma trabeculectomy.关键词
Fas/FasMcAb/小梁切除术/成纤维细胞/凋亡Key words
Fas/FasMcAb/Trabeculectomy/Fibroblast/Apoptosis引用本文复制引用
黎宗汉,谭思敏,柯毅,彭红娟..Fas及Fas-L对小梁切除术后成纤维细胞的凋亡研究[J].中国医学创新,2015,(10):18-20,3.基金项目
湛江市科技计划项目资助 （）
