中国医学创新Issue(14)：60-62,63,4.DOI:10.3969/j.issn.1674-4985.2015.14.020
结肠癌组织IL-10和IFN-γ mRNA的表达及其临床意义
Expression and Clinical Significance of IL-10 and IFN-γ mRNA in Colon Cancer Tissue
邓菲丹 1罗建业2
作者信息
- 1. 广东省深圳市福田区人民医院锦龙社区健康服务中心 广东 深圳 518031
- 2. 广东省深圳市坪山新区人民医院中集社区健康服务中心
- 折叠
摘要
Abstract
Objective:To investigate the gene expression of interleukin-10(IL-10) and interferon-γ(IFN-γ) colon tissue and methylation status of the promoter region.Method:Randomly selected from January 2011 to June 2014 colon cancer patient’s tissue 80 cases of colon cancer group, 80 cases of corresponding adjacent tissues as adjacent group, ad 100 cases of normal healthy colon tissue as the control group.Using bisulfite sequencing method (BSP),methylation specific PCR(MSP) and real-time quantitative PCR (RT-PCR) detection of promoter methylation of three groups of patients with colonic tissue IL-10 and IFN-γ gene and mRNA expression status.Result:Comparison of the three groups in the tissue IL-10 and IFN-γ mRNA, the differences were statistically significant(P<0.05).Colon cancer group and adjacent group of IL-10 mRNA were higher than the control group(P<0.05),IFN-γ mRNA were lower than in the control group(P<0.05).Three groups of IL-10 gene promoter region-110,-185 sites methylation rates,the difference was statistically significant(P<0.05).Colon cancer group and adjacent group organization of IL-10 gene promoter region -110 were higher than the control group(P<0.05),colon cancer group IL-10 gene promoter region-185 were lower than in the control group(P<0.05);IFN-γ gene promoter methylation of the colon cancer group>the adjacent group>the control group, the differences were statistically significant(P<0.05).Conclusion:IL-10 and IFN-γ gene promoter methylation status affect its expression in colon cancer, colon cancer patients in TH2 polarization state, closely related to the development of colon cancer.关键词
结肠肿瘤/DNA甲基化/组织/干扰素-γ/白细胞介素-10Key words
Colon cancer/DNA methylation/Tissue/Interferon-γ/Interleukin-10引用本文复制引用
邓菲丹,罗建业..结肠癌组织IL-10和IFN-γ mRNA的表达及其临床意义[J].中国医学创新,2015,(14):60-62,63,4.
