中国医学创新Issue(15)：19-22,4.DOI:10.3969/j.issn.1674-4985.2015.15.007
去卵巢致大鼠腰椎间盘退变模型初探
A Preliminary Discussion on Lumbar Disc Degeneration Model of Rat by Ovariectomizing
摘要
Abstract
Objective:To observe effects of removing ovary on lumbar intervertebral disc, establishing rat model of lumbar intervetebral disc degeneration.Method:8-month-old healthy female SD rats were randomly divided into blank group, sham operation group and model group, 10 rats in each group.The blank group were subjected to no disposal. Rats of the sham operation group only underwent the resection of a bit fat.The model group underwent the resection of bilateral ovaries.all the rats were fed under the same conditions, All rats were sacrificed after feeding 13 weeks, the histomorphology of lumbar disc, notochord cells and the changes of the ultrastructure of nucleus pulposus cells were observed.Result:Tissue morphological grading and notochord cells quantity:there were significant differences between the model group and blank group (P<0.05), the difference in the blank group and the sham operation group was not significant(P>0.05).Level of degenerative nucleus pulposus cells observed under electron microscope:model group > blank group, It had no significant difference between the blank group and the sham operation group.Conclusion:Rat model of lumbar intervetebral disc degeneration was established preliminary through ovary resection of female rats.关键词
腰椎椎间盘退变/去卵巢/动物模型Key words
Lumbar disc degeneration/Ovariectomizing/Animal model引用本文复制引用
冯帅华,吴官保,杨届,尹青,李勇敏,武海兵,刘泽庚..去卵巢致大鼠腰椎间盘退变模型初探[J].中国医学创新,2015,(15):19-22,4.基金项目
湖南省自然科学基金（联合基金）资助项目 （）
