中国医学创新Issue(21)：16-18,3.DOI:10.3969/j.issn.1674-4985.2015.21.006
REM-PCL对犬缺血再灌注心肌线粒体抗氧化能力的影响
Effects of REM-PCL on the Ability of Anti-lipid Peroxidation of Canine Myocardial Mitochondrial during Ischemia/Reperfusion
摘要
Abstract
Objective:To observe the effect of remifentanil-poly-caprolactone(REM-PCL)on the ability of anti-lipid peroxidation of canine myocardial mitochondrial during cardiopulmonary bypass(CPB).Method:Twelve healthy dogs undergoing CPB were randomly allocated into control group(group C,n=6)and REM-PCL pretreatment group(group RP, n=6).All the dogs were anesthesitized with 2.5% sodium pentobarbitone 25mg/kg.After tracheal intubation,the myocardial ischemia-reperfusion injury model during CPB was made by declamping(DC)after aortic cross-clamping(AC)for 60 min.In group P,REM-PCL 0.2 mg/kg was administered intravenously before CPB while in group C physiological saline was given instead of REM-PCL.Myocardial mitochondria were isolated before CPB,60min after AC,30 min and 60 min after DC for determination of mitochondrial swelling degree(MSD), malondialdehyde(MDA),glutathione peroxidase(GSH-PX),superoxide dismutase (SOD)and total anti-oxidation capacity(T-AOC).Result:The myocardial mitochondria of MSD and MDA were increased while GSH-PX,SOD and T-AOC were decreased after AC as compared to the value before CPB in both groups(P<0.01). MSD and MDA were increased while GSH-PX,SOD and T-AOC were decreased after DC as compared to the value before CPB in group C(P<0.01).The myocardial mitochondria of group RP at 60min after DC had not significant differences in content of MSD,MDA,GSH-PX,SODand T-AOC(P>0.05).MSD and MDA were distinctly higher while GSH-PX,SOD and T-AOC were distinctly lower at 60min after AC and after DC in group RP than those in group C(P<0.01).Conclusion:Pretreatment with REM-PCL is effective in improving the anti-oxidation ability of myocardial mitochondria and attenuate myocardial mitochondrial injury.关键词
瑞芬太尼聚己内酯/线粒体/体外循环Key words
Remifentanil-poly-caprolactone/Mitochondria/Cardiopulmonary bypass引用本文复制引用
方华,杨淼,张伟晶,章建平,张竞超,章放香..REM-PCL对犬缺血再灌注心肌线粒体抗氧化能力的影响[J].中国医学创新,2015,(21):16-18,3.基金项目
贵州省科技厅基金资助项目（黔科合LH字[2014]7021号）；贵州省科技厅基金资助项目 （）
