中国医学前沿杂志（电子版）Issue(6)：26-29,4.
心肌梗死后MicroRNA-24调控心肌细胞凋亡的作用研究
MicroRNA-24 after myocardial infarction regulating the clinical research of myocardial cell apoptosis
摘要
Abstract
Objective In mice after myocardial infarction (MicroRNA-24, miR-24) study of the effects of the expression and regulation of myocardial cell apoptosis. Method Established mouse model of myocardial infarction, using real-time quantitative polymerase chain reaction (qRT-PCR) quantitative detection of miR-24 expression after myocardial infarction in myocardial tissue;construct the lentivirus carrying miR-24 and liposome, on myocardial infarction tissues were cultured by local injection of lentiviral transfection of miR-24 and myocardial cells transfected with miR-24;measured and compared mice cardiac function and the area of myocardial infarction. Result QRT-PCR detection showed, ligation group in experiment ifrst, second week, the expression level of miR-24 in myocardial infarction area and the surrounding region in the fall, and the sham operation group, the expression level of comparative difference was signiifcant (P<0.05). Upregulation of miR-24 myocardial infarction area and the surroundingarea in the fourth week, restored to the level of sham operation group, the expression level of miR-24 between the two groups had no signiifcant difference (P>0.05);transfection of lentivirus 3 days later, using the method of qRT-PCR quantitative myocardial infarction local miR-24 expression level, results showed elevated expression the levels of myocardial transfection group mice miR-24 local miR-24, compared with the blank lentivirus transfection group difference in miR-24 expression was significant (P < 0.05); blank lentivirus transfection group and miR-24 transfected group compared with the sham operation group, LVEDD and LVESD were increased, LVEF and LVFS was decreased;level decreased after transfection of LVEDD, but the difference between the blanklentivirus transfection group and miR-24 transfected group was not signiifcant (P>0.05), LVESD after transfection, reduce differences, between the two groups was signiifcant (P<0.05), the expression level in miR-24 transfected group and blank lentivirus transfection group, LVEF and LVFS increased, the difference was signiifcant (P<0.05);differences between the 2 week blank lentivirus transfection group, miR-24 transfection group, model group of myocardial infarction area had signiifcant difference (P<0.05), after two two shows a comparison between the miR-24 transfection group and blank lentivirustransfection group area difference was signiifcant (P < 0.05). Conclusion The myocardial tissue after myocardial infarction, Micro RNA-24 lower expression, can inhibit cell apoptosis after infarction, improve myocardial function.关键词
心肌梗死/MicroRNA-24调控/心肌细胞凋亡Key words
Myocardial infarction/MicroRNA-24 regulation/Myocardial apoptosis引用本文复制引用
王磊,韩玮,罗建平,刘惠亮..心肌梗死后MicroRNA-24调控心肌细胞凋亡的作用研究[J].中国医学前沿杂志（电子版）,2014,(6):26-29,4.基金项目
武警总医院院级基金 （）
