中国医学前沿杂志（电子版）Issue(7)：17-19,3.
LiCl通过抑制GSK-3β的活性防治AD的研究
LiCl inhibits the activity of GSK-3βto prevent AD
王淑敏 1陈林林 1王伟 1梁文娜 1常立国1
作者信息
- 1. 聊城市第三人民医院 神经内科，山东 聊城 252000
- 折叠
摘要
Abstract
Objective To explore the mechanisma of LiCl which plays a role in protecting cells through regulating the activity of GSK-3β. Method Plasmids pBACE1-mychis and pAPPswe were transfected into SHSY5Y cells by LipofectaminTM2000. The cells were treated with LiCl and then were collected to determine the protein levels of GSK-3β,β-catenin and CyclinD1by Western blot.MTS, Brdu detect cell proliferation. Result Western blot results showed that in the cells, LiCl inhibited the expression of GSK-3β, and promoted the expression ofβ-catenin, CyclinD1. LiCl signiifcantly inhibited the growth of cells transfected. SHSY5Y. MTS inhibition rate was 23.45%, and Brdu inhibition rate was 22.58%. Conclusion GSK-3βis a potential target for treating with AD. LiCl can prevent AD through inhibiting the activity of GSK-3β.关键词
阿尔茨海默病/LiCl/糖原合成酶激酶-3βKey words
Alzheimer′s disease/LiCl/GSK-3β引用本文复制引用
王淑敏,陈林林,王伟,梁文娜,常立国..LiCl通过抑制GSK-3β的活性防治AD的研究[J].中国医学前沿杂志（电子版）,2014,(7):17-19,3.
