中国医学前沿杂志（电子版）Issue(2)：6-10,5.
NGS技术检测中国原发性肺癌患者的EGFR、KRAS、BRAF和PIK3CA基因突变
NGS detection for Chinese primary lung cancer patients gene mutation on EGFR, KRAS, BRAF and PIK3CA
摘要
Abstract
Objective To evaluate the feasibility of application of next generation sequencing (NGS) technology in clinical molecular diagnosis of lung cancer. Method The NGS platform-Ion Torrent was utilized in this study to examine the mutational status of EGFR, KRAS, BRAF and PIK3CA in 30 cases of lung cancer. The NGS results were compared with qPCR results. Result The technical parameters of NGS method were stable and the NGS results were entirely consistent with qPCR results (100%). Conclusion After further technical validation of more clinical samples, NGS technology is expected to be applied broadly in clinical setting. It is believed to elevate the molecular diagnosis and individualized treatment of lung cancer to a new level.关键词
下一代测序/原发性肺癌/基因突变/临床分子诊断Key words
Next generation sequencing/Lung cancer/Gene mutation/Clinical molecular diagnosis引用本文复制引用
支修益,胡牧,王鑫..NGS技术检测中国原发性肺癌患者的EGFR、KRAS、BRAF和PIK3CA基因突变[J].中国医学前沿杂志（电子版）,2015,(2):6-10,5.基金项目
首都医学发展科研基金 （）
