中国癌症防治杂志Issue(2)：121-126,6.DOI:10.3969/j.issn.1674-5671.2014.02.05
沉默CDKN1A基因对鼻咽癌放射抗拒性CNE-2R细胞放射敏感性的影响
Effect of silencing gene CDKN1A on radiosensitivity of radioresistant human nasopharyngeal carcinoma CNE-2R cell
摘要
Abstract
Objective To investigate the relationship between the CDKN1A gene expression and the change of radiosensitivity of human nasopharyngeal carcinoma. Methods Lentiviralvector-mediated RNA interference(LV-CDKN1A-RNAi)targeting was constructed and transfected it to CNE-2R cells.CNE-2R cells transfected with LV-CDKN1A-RNAi were taken as experimental group,those transfectedwith control virus and without any treatment were taken as control group and blank group.The proliferation inhibitory rate was measured by CCK-8 assay.Colony formation assay was performed to determine the radiosensitizing effect.Cell cycle distribution was analyzed by flow cytometry. Results The stably silenced expression of CDKN1A CNE-2R cell line was established.CCK-8 assay showed that the growth of CNE-2R cell of the experimental group was inhibited after 6 Gy irradiating,which was closely related to growth time.The results of colony formation assay showed that the radiosensitivity of CNE-2R cell of the experimental group was more enhance(SER=1.24)than the other groups.Comparing with control group,cell cycle distribution showed that the cell distribution of G0/G1 and G2/M cycle in the experiment group was significantly changed before and after 6 Gy irradiating. Conclusions Silencing gene CDKN1A may enhance the radiosensitivity of CNE-2R cells of human nasopharyngeal carcinoma,which maybe used as a thera-peutic target for nasopharyngeal carcinoma.关键词
鼻咽肿瘤/放射敏感性/CDKN1A/转染Key words
Nasopharyngeal neoplasm/Radioresistance/CDKN1A/Transfect分类
医药卫生引用本文复制引用
马嘉林,郭亚,曲颂,赵伟,李龄,苏芳,李烨,葛莲英,朱小东..沉默CDKN1A基因对鼻咽癌放射抗拒性CNE-2R细胞放射敏感性的影响[J].中国癌症防治杂志,2014,(2):121-126,6.基金项目
国家自然科学基金资助项目（30860329）；广西自然科学基金资助项目（桂科自0832229）；广西高等学校重大科研基金资助项目（201101D004）；广西卫生厅科研基金资助项目 （）
