中国癌症防治杂志Issue(3)：166-172,7.DOI:10.3969/j.issn.1674-5671.2015.03.06
大黄素衍生物A抑制卵巢癌淋巴结定向高转移细胞的增殖、迁移及对Rac1、CDC42表达的调控作用
Emodin Derivative A regulates Rac1 and CDC42 to inhibit proliferation and migration of ovarian cancer cells with lymph node-oriented metastasis
摘要
Abstract
Objective To study the effects of Emodin Derivative A on SKOV3-pm4 ovarian cancer cells with lymph node-oriented metastasis,particularly on expression of Rac1 and CDC42 mRNA and protein. Methods SKOV3-pm4 cells were treated for 24 h with different concentrations of Emodin Derivative A in the presence or absence of Rac1 inhibitor or activator. Cell proliferation was assessed by MTT assay,cell migration,by scratch assay;Rac1 and CDC42 expression,by RT-PCR and Western blotting;and distribution of these two proteins,by immunofluorescence. Results Emodin Derivative A at doses of 5-50 mg/L inhibits the proliferation of SKOV3-pm4 cells (IC50 34.46 mg/L). Rac1 and CDC42 protein were present throughout the cytoplasm,though Rac1 staining was denser around the nucleus than in other parts of the cell. Expression of Rac1 mRNA and protein decreased as the dose of Emodin Derivative A increased. CDC42 expression,however,did not change significantly with drug dose. Emodin Derivative A at a dose of 4 mg/L inhibited cell migration. Conclusions Emodin Derivative A can inhibit migration and proliferation of SKOV3-pm4 cells,and the drug may act by targeting Rac1.关键词
卵巢肿瘤/大黄素衍生物A/增殖/迁移/Rac1/CDC42Key words
Ovarian neoplasm/Emodin Derivative A/Proliferation/Migration/Rac1/CDC42分类
医药卫生引用本文复制引用
李鸿,唐敏,谢一泓,侯华新,黎丹戎..大黄素衍生物A抑制卵巢癌淋巴结定向高转移细胞的增殖、迁移及对Rac1、CDC42表达的调控作用[J].中国癌症防治杂志,2015,(3):166-172,7.基金项目
国家自然科学基金资助项目（81360502）；广西自然科学基金资助项目 （）
