中国癌症防治杂志Issue(3)：177-181,5.DOI:10.3969/j.issn.1674-5671.2015.03.08
人白细胞介素18对肺癌A549细胞生物学功能的影响
Influence of IL-18 on biological function of human lung cancer cell A549
摘要
Abstract
Objective To investigate the biological functions of IL-18 on the human lung cancer cell A549,including its role in cancer-related processes. Methods A plasmid expressing IL-18 or a control plasmid were transfected into the A549 human lung cancer cell line,and antibiotic selection was used to identify stable transfectants. The resulting respective cell lines,IL18-A549 and NC-A549,were compared in terms of proliferation,apoptosis,movement,invasion and migration. Results The IL18-A549 cell line stably expressed IL-18,and comparison with the NC-A549 cell line suggested that IL-18 promotes proliferation (P<0.05). The rate of healing in a wound healing assay was significantly higher for IL18-A549 cells (62.0±5.7)% than for control cells (31.2±3.7)%(P<0.05),and the percentages of cells showing migration and invasion in a transwell assay were significantly higher for IL18-A549 (48%and 33%)than for controls (9%and 11%;P<0.05). Flow cytometry showed similar rates of early,late and total apoptosis between IL-18 transfectants and control cells. Conclusion IL-18 promotes proliferation,movement,invasion and migra-tion of A549 cells,and may play a key role in human lung cancer. Inhibiting IL-18 function may be useful for treating lung cancer.关键词
肺肿瘤/人白细胞介素18/生物学功能Key words
Lung neoplasm/Interleukin 18/Biological function分类
医药卫生引用本文复制引用
梁振鑫,罗少仕,欧阳轶强,梁锦宁,周怡..人白细胞介素18对肺癌A549细胞生物学功能的影响[J].中国癌症防治杂志,2015,(3):177-181,5.基金项目
广西自然科学基金资助项目 （）
