中国癌症防治杂志Issue(3)：182-186,187,6.DOI:10.3969/j.issn.1674-5671.2015.03.09
应用基因表达谱芯片技术研究Rituximab的耐药机制
Preliminary cDNA microarray studies of Rituximab resistance mechanisms
摘要
Abstract
Objective In this study,we aimed to use cDNA microarrays to identify differentially expressed genes and activation or inhibition of signaling pathways that may account for the phenotype of rituximab-resistant cell lines. Methods Rituximab-resistant B-cell non-Hodgkin’s lymphoma cell lines were established,and cDNA microarray analysis was performed on the resistant and parental cell lines. Bioinformatics analysis was carried out using the KEGG database and DAVID software. Results We identified 70 genes that were up-regulated and 42 that were down-regulated in both Jeko-1/R and Raji/R resistant cell lines compared to parental lines. KEGG pathway analysis suggested that the MAPK signaling pathway is significantly more active in Jeko-1/R and Raji/R cells. GO term analysis of differentially expressed genes suggested that rituximab-resistant cells show the characteristics of“anti-apoptosis”,“promoting proliferation”and“blood vessel development”. Conclusion Our results suggest that rituximab resistance is most closely associated with the MAPK signaling pathway,which may act to inhibit apoptosis and promote proliferation and vascular development. These findings provide a theoretical basis for predicting and overcoming rituximab resistance in the clinical setting.关键词
淋巴瘤/Rituximab/药物耐药/基因表达谱芯片/MAPK信号通路/生物信息学Key words
Lymphoma/Rituximab/Drug resistance/cDNA microarray/MAPK signaling pathway/Bioinformatics分类
医药卫生引用本文复制引用
潘梅芳,岑洪,谭晓虹,王明月..应用基因表达谱芯片技术研究Rituximab的耐药机制[J].中国癌症防治杂志,2015,(3):182-186,187,6.基金项目
国家自然科学基金资助项目（81160298）；广西医疗卫生重点科研课题（重2011077）；广西医疗卫生适宜技术研究与开发课题 （）
