中国医学装备Issue(9)：9-11,3.DOI:10.3969/J.ISSN.1672-8270.2014.09.004
增强型绿色荧光蛋白融合人核糖核酸酶抑制因子包装细胞系的建立及鉴定
Identification of the expression of recombinated plasmid pLNCX-EGFP-C1-hRI in PA317 ;packaging cell line
摘要
Abstract
Objective: To identify the expression of plasmid pLNCX-EGFP-C1-hRI targeting the gene of Human ribonuclease inhibitor (hRI) in PA317 cells which is capable of expression in mammalian cells. Methods: The vector of pLNCX-EGFP-C1-hRI was transfected into PA317 cells by Lipofectamine 2000 and then the expression of recombinated plasmid was verified in living cells by observing the transcription level of egfp-hRI fusion gene mRNA with RT-PCR method and the expression level of egfp-fusion hRI protein with western blotting method respectively. Results:Both RT-PCR and western blotting showed the egfp-hRI fusion gene was obviously expression in PA317 cells. Conclusion: The plasmid of pLNCX-EGFP-C1-hRI targeting hRI is successfully constructed and the protein of hRI can be expressed in PA317 cells correctly.关键词
人核糖核酸酶抑制因子/增强型绿色荧光蛋白/鉴定Key words
Human ribonuclease inhibitor/Enhanced green fluorescent protein/Identification分类
医药卫生引用本文复制引用
丁宁,李坤,常明..增强型绿色荧光蛋白融合人核糖核酸酶抑制因子包装细胞系的建立及鉴定[J].中国医学装备,2014,(9):9-11,3.基金项目
辽宁省教育厅科研课题(L2011219)“阻断内源性Angiogenin释放在抗肝癌血管生成中的应用研究” （L2011219）
