中国医学装备Issue(10)：1-4,5,5.DOI:10.3969/J.ISSN.1672-8270.2014.10.001
免疫传感器在癌胚抗原检测中的构建与应用研究
Research on immunosensor for the determination of carcinoembryonic antigen and its application
摘要
Abstract
Objective: To design immunosensor for the determination of Carcinoembryonic Antigen(CEA) and be used in the piont-of-care test of the concentration of CEA. Methods:The immunosensor was developed by modifying multi-wall carbon nanotubes(MWNTs)which dispersed in N, N-dimethyl-formamide(DMF)on the Screen-Printed Electrodes (SPE), then Chitosan-NanoCeO2 were immobilized self-assembly. And then the antibody of CEA (anti-CEA) was absorbed on the surface of NanoCeO2 monolayer. Finally, Albumin from bovine serum (BSA) blocked the non-specific adsorption site.The modification process was characterized by transmisCeOn electronmicroscope(TEM)and cyclic voltammetry(CV). The factors possibly influenced the performance of the proposed immunosensor were studied in detail. Results:Under optimal conditions,the obtained immunosensor exhibited good electrochemical behavior to CEA in a linear range of 0 to 80 ng/ml (r=0.99825), with a detection limit of 0.08 ng/ml. Conclusion: The immunosensor prepared using this method has advantages of good stability, regeneration, high sensitivity and specificity. Thus, the method can be used for clinical application.关键词
癌胚抗原/丝网印刷电极/多壁碳纳米管/纳米铈Key words
Carcinoembryonic antigen/Screen-printed electrodes/Multi-wall carbon nanotubes/NanoCeO2分类
医药卫生引用本文复制引用
马霄,莫利明,虞成,逯岭松,杨攀..免疫传感器在癌胚抗原检测中的构建与应用研究[J].中国医学装备,2014,(10):1-4,5,5.基金项目
国家自然科学基金(81301514)“基于DNA甲基化多指标联合检测卵巢癌的新方法研究”；浙江省科研项目基金(2011C37027)“基于双纳米生物传感技术的MPO即时检测系统” （81301514）
