中国药业Issue(11)：4-4,5,2.
高效液相色谱法测定月季花中金丝桃苷含量
Determination of Hyperin Content in Rosa chinensisJacq by HPLC
崔慧芳 1常欣1
作者信息
- 1. 浙江省宁波市药品检验所，浙江 宁波 315048
- 折叠
摘要
Abstract
Objective To establish a HPLC method for determining the hyPerin content in Rosa chinensis jacq. Methods The Agilent C18 column ( 100 mm × 4. 6 mm, 2. 7 μm ) was adoPted with the mobile of acetonitrile-0. 05% PhosPhoric acid ( 16 : 84 ); the detection wavelength was set at 360 nm with flow rate of 0. 5 mL/min. Results A good linearity relation was observed between the samPle size of hyPerin during the range of 0. 047 68-0. 476 80μg with the Peak area. The average recovery rate of hyPerin was 97. 22%, RSD=0. 89% ( n=9 ) . Conclusion This method is raPid, convenient, stable, reliable and strongly sPecific for the dermination of hyPerin in Rosa chinensis jacq.关键词
高效液相色谱法/月季花/金丝桃苷/含量测定Key words
HPLC/rosa chinensis jacq/hyPerin/content determination分类
医药卫生引用本文复制引用
崔慧芳,常欣..高效液相色谱法测定月季花中金丝桃苷含量[J].中国药业,2014,(11):4-4,5,2.
