中国药业Issue(14)：41-42,2.
芦荟大黄素对人永生化角质形成细胞增殖和凋亡作用研究
Effect of Aloe Emodin on Proliferation and Apoptosis of HaCaT Cells
摘要
Abstract
Objective To study the growth inhibition effect of aloe emodin(AE)on human immotalized keratinocytes(HaCaT)and its apoptotic induction ability,and to explore its possible mechanism in treating psoriasis. Methods The MTT assay was used to detect the proliferation inhibition effect of aloe emodin on the HaCaT cells. The morphological changes were observed by using the inverted micro-scope. The flow cytometry was used to detect the change of cell cycle and apoptosis. Results AE in the range of 20 ~ 80 μg / mL could inhibit the proliferation of HaCaT cells on a dose - dependent manner. The sparse cells,slowed growth and elongated cell shape were observed under microscopy. And the cell cycle was arrested in the S phase after AE treatment,thus apoptosis occurred. Conclusion Aloe emodin inhibits HaCaT cells proliferation and induces HaCaT cells apoptosis,which can be used in the treatment of psoriasis.关键词
芦荟大黄素/人永生化角质形成细胞/银屑病Key words
aloe emodin/HaCaT cells/psoriasis分类
医药卫生引用本文复制引用
曾德贵,莫衍石,刘荣丰,杨春燕,林世平,陈小谊,兰柳波..芦荟大黄素对人永生化角质形成细胞增殖和凋亡作用研究[J].中国药业,2014,(14):41-42,2.基金项目
广东省深圳市科技计划项目，项目编号201202105。 （）
