中国药业Issue(15)：23-24,2.
胡椒碱对乳腺癌阿霉素耐药细胞株 MCF -7/ADM 细胞的逆转作用
Reversal Effect of Piperine on Adriamycin - Resistant MCF - 7/ADM Cells of Breast Cancer
摘要
Abstract
Objective To explore the reversal effect and mechanism of piperine on the multidrug adriamycin(ADM) - resistance cell line MCF - 7 / ADM of human breast cancer. Methods The effect of piperine combined with ADM on growth and proliferation of drug - re-sistant MCF - 7 / ADM cells were observed by using the CCK - 8 assay. The expression levels of P - glycoprotein (P - gp) in MCF - 7 / ADM cells treated with piperine were detected by Western blot. Results The drug resistance reversal fold in the experimental groups with 60,80,100 μmol / L of piperine was 1. 62,2. 08,3. 78 times respectively. Piperine could significantly inhibit the expression of P - gp on the cytomembrane of MCF - 7 / ADM cells and enhanced the cellular sensitivity to ADM. Conclusion Piperine and adriamyci co - acting on MCF - 7 / ADM cells can increase the cellular sensitivity to ADM. Piperine can partially induce the reversal of drug resistance of MCF - 7 / ADM cells, its mechanism may be related with the down - regulation of the P - gp expression.关键词
胡椒碱/多药耐药/P-糖蛋白/乳腺癌阿霉素耐药细胞株MCF-7/ADM细胞Key words
piperine/multidrug resistance/P-glycoprotein/MCF-7/ADM cell分类
医药卫生引用本文复制引用
赖艳,温悦,卢来春..胡椒碱对乳腺癌阿霉素耐药细胞株 MCF -7/ADM 细胞的逆转作用[J].中国药业,2014,(15):23-24,2.基金项目
重庆市卫生局中医药资助课题，项目编号2011-2-115。 （）
