中国药业Issue(16)：41-42,43,3.
甘草中异甘草素和甘草素含量测定方法研究
Determination of Content of Isoliquiritigenin and Liquiritigenin
摘要
Abstract
Objective To establish the method for simultaneously determining the isoliquiritigenin and liquiritigenin contents in gly-cyrrhiza uralensis Fisch. Methods The isocratic elution and the detection wavelength time sequence sampling by HPLC were adopt-ed. The mobile phase was methanol - water(45 : 55),the detection wavelength was 276 nm for 0 - 25 min and 360 mn for 25 - 70 min and the flow rate was 0. 8 mL. min - 1. Under these conditions,the isoliquiritigenin and liquiritigenin contents in glycyrrhiza uralensis Fisch were simultaneously determined. Results Isoliquiritigenin(0. 028 - 0. 28 μg)and liquiritigenin(0. 064 - 0. 64 μg)had good lin-ear relation. The average recovery rates of the two components were 97. 92% - 98. 27% with RSD of 1. 29% - 1. 67% . Conclusion The method is accurate and highly sensitive with good reproducibility and easy operation,and can provide the reference data for the e-valuation of glycyrrhiza uralensis Fisch medicinal material.关键词
甘草/异甘草素/甘草素Key words
glycyrrhiza uralensis Fisck/isoliquiritigenin/liquiritigenin分类
医药卫生引用本文复制引用
周娅静,常靖,石海燕,王青,马雪梅..甘草中异甘草素和甘草素含量测定方法研究[J].中国药业,2014,(16):41-42,43,3.基金项目
山西省科技攻关项目，项目编号20130313011-2和20130313006-3。 （）
